Tulsi - Ocimum sanctum: A herb for all reasons

Abstract

The predominant cause of global morbidity and mortality is lifestyle-related chronic diseases, many of which can be addressed through Ayurveda with its focus on healthy lifestyle practices and regular consumption of adaptogenic herbs. Of all the herbs used within Ayurveda, tulsi (Ocimum sanctum Linn) is preeminent, and scientific research is now confirming its beneficial effects. There is mounting evidence that tulsi can address physical, chemical, metabolic and psychological stress through a unique combination of pharmacological actions. Tulsi has been found to protect organs and tissues against chemical stress from industrial pollutants and heavy metals, and physical stress from prolonged physical exertion, ischemia, physical restraint and exposure to cold and excessive noise. Tulsi has also been shown to counter metabolic stress through normalization of blood glucose, blood pressure and lipid levels, and psychological stress through positive effects on memory and cognitive function and through its anxiolytic and anti-depressant properties. Tulsi's broad-spectrum antimicrobial activity, which includes activity against a range of human and animal pathogens, suggests it can be used as a hand sanitizer, mouthwash and water purifier as well as in animal rearing, wound healing, the preservation of food stuffs and herbal raw materials and traveler's health. Cultivation of tulsi plants has both spiritual and practical significance that connects the grower to the creative powers of nature, and organic cultivation offers solutions for food security, rural poverty, hunger, environmental degradation and climate change. The use of tulsi in daily rituals is a testament to Ayurvedic wisdom and provides an example of ancient knowledge offering solutions to modern problems.

Main article text

INTRODUCTION

Diseases of modern life

Despite the many wonders of science and industry, modern life is fraught with stress. Mobile devices and the web have vastly increased the pace of life so that many people feel that they are now drowning in an ever-expanding ocean of data, while industrial agriculture has burdened us with increasing exposure to unhealthy processed and packaged food and a plethora of pesticides, food packaging materials and other toxic industrial chemicals. Urban dwellers are also faced with increasing wealth inequality, social isolation, excessive noise, air, water and soil pollution and disconnection from nature. Thus, while industrialization has led to longer lifespans and vast increases in human populations, it is now recognized that the greatest causes of death and disease on the planet are preventable lifestyle-related chronic diseases.[1]

We are in the midst of a global pandemic of obesity, diabetes, cancer, dementia, depression and other chronic diseases caused by modern lifestyles and their associated lack of physical activity, high intake of sugar, fat, salt, alcohol and tobacco and exposure to a toxic cocktail of industrial chemicals. The solutions to this current health crisis are therefore more likely to be found in the homes and behaviors of individuals than in medical clinics, hospital or pharmacies.

Ayurveda and lifestyle medicine

As a science of life and the world's oldest medical system, Ayurveda has a holistic approach to health and disease that focuses on preserving and promoting good health and preventing disease through healthy lifestyle practices. These practices include consumption of fresh, minimally processed foods, the use of Rasayanas (formulas) that eradicate ageing and disease, sophisticated detoxification practices and regular consumption of adaptogenic herbs that enhance the body's capacity to maintain balance in the midst of a variety of stressors.

Ayurveda's use of medicinal and culinary herbs draws upon India's incredible biodiversity with a variety that is unsurpassed by any medical system; yet, of all the herbs used, none has a status comparable to tulsi or holy basil (Ocimum sanctum).

Tulsi: A potent adaptogen

Tulsi is an aromatic shrub in the basil family Lamiaceae (tribe ocimeae) that is thought to have originated in north central India and now grows native throughout the eastern world tropics.[2] Within Ayurveda, tulsi is known as “The Incomparable One,” “Mother Medicine of Nature” and “The Queen of Herbs,” and is revered as an “elixir of life” that is without equal for both its medicinal and spiritual properties.[3] Within India, tulsi has been adopted into spiritual rituals and lifestyle practices that provide a vast array of health benefits that are just beginning to be confirmed by modern science. This emerging science on tulsi, which reinforces ancient Ayurvedic wisdom, suggests that tulsi is a tonic for the body, mind and spirit that offers solutions to many modern day health problems.

Tulsi is perhaps one of the best examples of Ayurveda's holistic lifestyle approach to health. Tulsi tastes hot and bitter and is said to penetrate the deep tissues, dry tissue secretions and normalize kapha and vata. Daily consumption of tulsi is said to prevent disease, promote general health, wellbeing and longevity and assist in dealing with the stresses of daily life. Tulsi is also credited with giving luster to the complexion, sweetness to the voice and fostering beauty, intelligence, stamina and a calm emotional disposition.[3456] In addition to these health-promoting properties, tulsi is recommended as a treatment for a range of conditions including anxiety, cough, asthma, diarrhea, fever, dysentery, arthritis, eye diseases, otalgia, indigestion, hiccups, vomiting, gastric, cardiac and genitourinary disorders, back pain, skin diseases, ringworm, insect, snake and scorpion bites and malaria.[3567]

Considered as a potent adaptogen, tulsi has a unique combination of pharmacological actions that promote wellbeing and resilience. While the concept of an “adaptogen,” or herb that helps with the adaptation to stress and the promotion of homeostasis, is not widely used in Western medicine, Western science has revealed that tulsi does indeed possess many pharmacological actions that fulfill this purpose.

The medicinal properties of tulsi have been studied in hundreds of scientific studies including in vitro, animal and human experiments. These studies reveal that tulsi has a unique combination of actions that include: Antimicrobial (including antibacterial, antiviral, antifungal, antiprotozoal, antimalarial, anthelmintic), mosquito repellent, anti-diarrheal, anti-oxidant, anti-cataract, anti-inflammatory, chemopreventive, radioprotective, hepato-protective, neuro-protective, cardio-protective, anti-diabetic, anti-hypercholesterolemia, anti-hypertensive, anti-carcinogenic, analgesic, anti-pyretic, anti-allergic, immunomodulatory, central nervous system depressant, memory enhancement, anti-asthmatic, anti-tussive, diaphoretic, anti-thyroid, anti-fertility, anti-ulcer, anti-emetic, anti-spasmodic, anti-arthritic, adaptogenic, anti-stress, anti-cataract, anti-leukodermal and anti-coagulant activities.[4567] These pharmacological actions help the body and mind cope with a wide range of chemical, physical, infectious and emotional stresses and restore physiological and psychological function.

Protection and detoxification

Many of the physiological benefits of tulsi can be attributed to its ability to assist with the body's internal housekeeping and protection of the body from toxin-induced damage. These functions are often attributed to tulsi's high content of phenolic compounds and anti-oxidant properties, with Krishna tulsi (black/purple variety) having a higher phenolic content and anti-oxidant capacity than white Vana (wild) tulsi.[8]

Laboratory studies have shown that tulsi protects against toxic chemical-induced injury by increasing the body's levels of anti-oxidant molecules such as glutathione and enhancing the activity of anti-oxidant enzymes such as superoxide dismutase and catalase, which protect cellular organelles and membranes by mopping up damaging free radicals caused by lack of oxygen[9] and other toxic agents.[1011]

Tulsi also helps to prevent cancers caused by toxic compounds by reducing DNA damage[12] and inducing apoptosis in precancerous and cancerous cells, thereby reducing the growth of experimental tumors and enhancing survival.[1314] Furthermore, tulsi not only protects against the damage caused by toxic compounds, but also enables the body to more effectively transform and eliminate them by enhancing the activity of liver detoxification enzymes such as the cytochrome P450 enzymes, which deactivates toxic chemicals and enables them to be safely excreted.[15]

While these actions are vitally important for protecting against natural toxins produced within the body or by animals or plants, they are perhaps even more important in the modern age to protect against the vast range of pollutants, pesticides, pharmaceuticals, heavy metals, radiation and other industrial toxicants created from human activity.

Toxicant stress: Chemicals, heavy metals and radiation

The ability of tulsi to protect against the damaging effects of various toxicants has been documented in numerous experimental studies. These studies attest to the ability of tulsi to prevent liver, kidney and brain injury by protecting against the genetic, immune and cellular damage caused by pesticides, pharmaceuticals and industrial chemicals. Thus, tulsi has been shown to protect against the toxic effects of industrial chemicals such as butylparaben,[16] carbon tetrachloride,[17] copper sulfate[18] and ethanol,[19] and common pesticides such as rogor,[20] chlorpyrifos,[21] endosulfan[22] and lindane.[23] Tulsi has also been shown to protect against the toxic effects of many pharmaceuticals drugs including acetaminophen,[24] meloxicam,[25] paracetamol,[26] haloperidol[27] and anti-tubercular drugs.[28]

In addition to protecting against toxic chemicals, tulsi has also been shown to protect against the toxic effects of heavy metals such as lead, arsenic, cadmium, chromium and mercury[293031] and the toxic effects of radiation.[32333435] Tulsi exerts its radio-protective effects by scavenging free radicals and reducing the oxidative cellular and chromosomal damage induced by radiation,[33363738] thereby reducing organ damage and enhancing postradiation survival in experimental animals.[394041]

Physical stress

The actions that protect against the toxic effects of chemicals and radiation also help to address the toxic effects of many physical stressors. Prolonged physical exertion, physical restraint, exposure to cold and excessive noise disturb homeostasis by inducing physiological and metabolic stress. When the capacity to adapt to these stressors is exceeded, maladaptation occurs resulting in damage to biochemical pathways, organ function and health. Through enhancing various cellular and physiological adaptive functions, adaptogenic herbs such as tulsi are able to protect against this damage.

Studies using forced-swimming, restraint and cold-exposure stress in laboratory animals have shown that tulsi enhances aerobic metabolism, improves swimming time, reduces oxidative tissue damage and normalizes many physiological and biochemical parameters caused by physical stressors. Similarly, experimental studies have shown that tulsi helps reduce the effects of acute and chronic noise-induced stress in experimental animals, with enhancement of neurotransmitter and oxidative stress levels in discrete brain regions along with improved immune, ECG and corticosteroid responses.[42434445]

Metabolic stress

Metabolic stress due to poor diet, low physical activity and psychological stress is a prominent feature of modern lifestyles and “metabolic syndrome” is estimated to affect as much as one-third of modern populations. Metabolic syndrome, also known as “prediabetes” or “Syndrome X,” includes the “deadly quartet” of centripetal obesity, hypertension, high cholesterol and poor glucose regulation and is associated with chronic inflammation and a greater risk of diabetes, heart disease and stroke. While the exact causes of metabolic syndrome are still being debated, there is evidence to suggest that tulsi can assist in dealing with many features of metabolic syndrome and their consequences.

Numerous test tube and animal experiments as well as human clinical trials have shown that tulsi has anti-diabetic activity. Studies using diabetic laboratory animals have shown that tulsi can reduce blood glucose, correct abnormal lipid profiles[4647] and protect the liver and kidneys from the metabolic damage caused by high glucose levels.[48] Tulsi has also been shown to improve lipid profiles,[4950] prevent weight gain, hyperglycemia, hyperinsulinemia, hypertriglyceridemia and insulin resistance,[5152] and protect the organs and blood vessels from atherosclerosis[4953] in laboratory animals fed high-fat diets. Similarly, in human clinical trials, tulsi has shown to decrease glucose levels, improve blood pressure and lipid profiles[545556] and reduce many diabetic symptoms in patients with type 2 diabetes.[57]

The beneficial metabolic effects of tulsi are multiple and include protecting the liver, kidneys[49] and pancreatic islet cells from free radical damage;[58] enhancing liver bile acid synthesis[49] and reducing liver lipid synthesis;[52] enhancing insulin secretion[59] and action;[60] lowering cortisol levels;[61] and reducing inflammation. The anti-inflammatory action of tulsi, which has been observed in both acute and chronic inflammatory models in animals,[62636465] is attributed to tulsi's eugenol and linoleic acid content and the inhibition of both the cyclooxygenase and the lipoxygenase pathways of arachidonic acid metabolism.[6667] This enables tulsi to exert anti-inflammatory effects comparable to nonsteroidal anti-inflammatory drugs such as phenylbutazone,[68] ibuprofen, naproxen, aspirin[69] and indomethacin.[70]

Infection protection

Modern research has revealed that tulsi has anti-bacterial, anti-viral and anti-fungal activity[71] that includes activity against many pathogens responsible for human infections. Tulsi has also been shown to boost defenses against infective threats by enhancing immune responses in nonstressed and stressed animals[727374757677] and healthy humans.[78] While no human trials have been published, there is experimental evidence that tulsi may help in the treatment of various human bacterial infections including urinary tract infections,[79] skin and wound infections,[80] typhoid fever,[81] cholera,[82] tuberculosis,[83] gonorrhea,[84] acne,[85] herpes simplex,[86] leishmaniasis,[87] various pneumonias[88] and fungal infections,[89909192] as well as mosquito-borne diseases such as dengue, malaria and filariasis.[939495]

Tulsi has also been shown to be active against many animal pathogens, and this has led to tulsi being used in animal rearing to reduce infections in cows,[96] poultry,[97] goats,[98] fish[7677] and silkworms.[99] Tulsi's activity against water-borne and food-borne pathogens further suggests that it can be used in the preservation of food stuffs[100101102] and herbal raw materials[103] as well as for water purification[82] and as a hand sanitizer.[104]

Tulsi's broad-spectrum activity, which includes activity against Streptococcus mutans, the organism responsible for tooth decay, further suggests that it can be used as a herbal mouth wash for treating bad breath, gum disease and mouth ulcers.[105106] This has been confirmed in clinical trials that have demonstrated that rinsing with tulsi is as effective as 0.2% Chlorhexidine and Listerine in reducing the levels of Streptococcus mutans[107] and that a herbal mouthwash that includes tulsi is preferred for its taste and convenience.[108]

Tulsi's unique combination of antibacterial antioxidant, anti-inflammatory and analgesic activities also makes it useful in wound healing.[109110] This is supported by experimental evidence that has shown that tulsi can increase wound-breaking strength and accelerate wound healing in laboratory animals.[110111] Tulsi has also been shown to have anti-ulcer and ulcer-healing activity that has been observed in many different animal models including aspirin-, indomethacin-, alcohol-, histamine-, reserpine-, serotonin-, acetic acid-, meloxicam-, cold restraint-, pyloric ligation- and stress-induced ulceration models.[25112113114] This anti-ulcer activity is attributed to multiple actions including the reduction of offensive factors such as acid-pepsin secretion and lipid peroxidation and the enhancement of gastric defensive factors such as mucin secretion, cellular mucus and longevity of mucosal cells.[114115]

Mental stress

In addition to physical, toxic and infective stress, modern living is associated with heightened levels of psychological stress caused by the many demands and fast pace of modern life. This stress compounds the toxic effects of chemical pollutants and the constant fear of pervasive toxic chemicals can itself lead to even further stress and anxiety that may be just as toxic as the chemicals causing it. While the reality of daily chemical exposure cannot be denied, regular consumption of tulsi not only helps protect and detoxify the body's cells and organs, it can also help reduce toxic stress by relaxing and calming the mind and offering many psychological benefits including anti-depressant activity and positive effects on memory and cognitive function.

The psychotherapeutic properties of tulsi have been explored in various animal experiments that reveal that tulsi has anti-anxiety and anti-depressant properties,[116117118] with effects comparable to diazepam and antidepressants drugs.[119120] Animal studies further reveal that tulsi enhances memory and cognitive function[121122] and protects against aging-induced memory deficits.[123] Similarly, in human studies, tulsi has been observed to reduce stress, anxiety and depression,[124] with a 6-week, randomized, double-blind, placebo-controlled study reporting that tulsi significantly improves general stress scores, sexual and sleep problems and symptoms such as forgetfulness and exhaustion.[125]

While modern scientific studies suggest that tulsi is effective in treating a range of stressful conditions, within Ayurveda, tulsi is more commonly recommended as a preventive measure to enhance the ability to adapt to both psychological and physical stress and therefore prevent the development of stress-related diseases. To this end, many Ayurvedic practitioners recommend the regular consumption of tulsi tea as an essential lifestyle practice.

Liquid yoga

Regular consumption of tulsi tea may be compared with the regular practice of yoga, which can be considered “adaptogenic” through nurturing and nourishing the body -- mind -- spirit while fostering a sense of relaxation and wellbeing. In contrast, regular consumption of caffeinated beverages such a black and green tea (Camellia sinensis L.) and coffee (Coffea arabica L.) may be compared with more aerobic exercise, which confers health benefits through stimulation and activation.

Like yoga, tulsi has a calming effect that leads to clarity of thought, along with a more relaxed and calm disposition. The cognitive and memory-enhancing properties of tulsi therefore differ from those of caffeine-containing beverages such as coffee and tea, which heightens arousal and may cause physical and mental agitation. Furthermore, tulsi does not produce the same physical dependence as caffeine and can be safely consumed on a regular basis without the fear of withdrawal effects.

The drinking of tea and coffee has become an integral part of modern life and has been ritualized in many cultures to guide social interactions, set social agendas and invoke spiritual awareness. For example, sophisticated Asian tea ceremonies involve a whole set of rituals, tools and gestures that serve to transcend normal consciousness, while in the west the ritual of “afternoon tea” or “high tea” emphasizes the surroundings, equipment, manners and social circle. In less-formal situations, many people ritualize their morning cup of coffee and use the “meet-up for coffee” to arrange their social agendas, while the “tea break” is often built into the modern-day work routine. Yet, while tea and coffee have infiltrated their way into modern living, they have not yet attained the status that tulsi has within traditional Indian life.

Divine tulsi

In Hinduism, tulsi is worshipped as a goddess and every part of the tulsi plant is revered and considered sacred, including the leaves, stem, flower, root, seeds and oil. Even the surrounding soil, which has recently been found to harbor beneficial endophytic fungi,[126] is considered an aspect of the divine. As such, Hindi households are considered incomplete without a tulsi plant, typically in an ornate earthen pot situated in a courtyard where tulsi serves both practical and ceremonial purposes. For example, tulsi's distinct clove-like aroma arising from its high eugenol content serves to link the householder to the divine while also repelling mosquitoes, flies and other harmful insects. Tulsi is further integrated into daily life through evening and morning rituals and other spiritual and purification practices that can involve ingesting its leaves or consuming tulsi tea.

In addition to sanctifying the home, tulsi is used ceremonially in Hinduism and some Greek Orthodox Churches to create “holy water.” Tulsi wood or seeds are also used to make tulsi malas, which are strings of beads used to help the mind focus during meditation, chanting and devotional practices and therefore ceremonially connect mind, body and spirit. Tulsi has also been used in cities to combat air pollution and hundreds of thousands of tulsi plants have been planted around the Taj Mahal in Agra to help protect the iconic marble building from environmental pollution damage.[127]

Nature nurture

The cultivation and reverence for the tulsi plant in the home not only serves specific religious purposes it also directly connects the devotee with the creative power of nature. Connection with nature is profoundly healing and life-affirming; yet, the potential health, emotional, social and cognitive benefits of connection with nature are only just being realized in the west where disconnection from nature and “nature deficit” are common. A review of the scientific literature on the health benefits of connection to nature suggest that “access to nature plays a vital role in human health, wellbeing and development that has not been fully recognized,”[128] and there is now a global movement to reconnect people with nature that has arisen out of concerns over nature deficit, sedentary lifestyles, obesity, mental health issues, excessive use of electronic media, environmental degradation, wildlife conservation, sustainability and climate change.[129]

The placing of a living tulsi plant at the center of the household, therefore, has applicability beyond the realms of Hinduism and may play a useful role in addressing modern day issues through embodying the healing power of the natural world and serving as a constant connection to living nature.

Quality assurance and identification

Like any medicinal plant, optimal cultivation, harvesting, preservation and storage methods are required to preserve tulsi's medicinal value. For example, it is suggested that tulsi should be grown employing organic methods in rural areas free from environmental pollution.[3] This is supported by the finding of toxic elements at almost twice the concentration in tulsi leaves grown in polluted compared with unpolluted areas.[130]

It is also important to ensure the correct herb is used and that manufacturers adopt stringent quality assurance standards and processes. Concerns about product quality in European “tulsi” products have been raised by reports of a high frequency of substitution with surrogate herbs such as Ocimum basilicum L.[131] This may be addressed using high-performance liquid chromatography fingerprints and microscopic assays to ensure batch-to-batch quality and the safety and botanical integrity of standardised extracts of standardized extracts.[131132]

Tulsi as a vehicle of consciousness

Perhaps one of the greatest of tulsi's benefits in the modern world comes from its global distribution based on its cultivation using ethical, fair trade, organic and ecological farming practices. There is a growing realization that in order to tackle issues of food security, rural poverty, hunger, environmental degradation and climate change a shift in agriculture is needed from a “green revolution” to an “ecological intensification revolution. This has been highlighted in a recent United Nations document titled “Wake Up Before It's Too Late,” which calls for the global community to endorse and advocate for local solutions to toxicity, food insecurity and poverty, such as the use of organic and small-scale farming over the use of genetically modified organisms and monocultures.[133] While ecological farming methods are not specific to tulsi, they have been effectively applied to tulsi cultivation by Organic India Pvt. Ltd. This company, which was established as a “vehicle of consciousness,” works with thousands of organic tulsi farmers in India to produce a business ecology whereby rural Indian farmers gain their dignity and a healthy and sustainable livelihood while serving to nurture the land they live on and produce a range of teas that enable consumers around the world to access the benefits of tulsi.

CONCLUSION

Modern day scientific research into tulsi demonstrates the many psychological and physiological benefits from consuming tulsi and provides a testament to the wisdom inherent in Hinduism and Ayurveda, which celebrates tulsi as a plant that can be worshipped, ingested, made into tea and used for medicinal and spiritual purposes within daily life. In providing a focus for ethical, sustainable and ecological farming practices that provides a livelihood for thousands of farmers, the cultivation of tulsi goes beyond providing benefits for individuals and households and begins to address broader social, economic and environmental issues.

Footnotes

Source of Support: Nil

Conflict of Interest: The author receives remuneration as a consultant and advisor to Organic India Pvt. Ltd., which is a company that manufactures and distributes tulsi products. This article is the independent work of the author and Organic India did not have input into the article's content or the decision to publish it.

REFERENCES

1. World Health Organisation. Department of Chronic Diseases and Health Promotion. Preventing Chronic Diseases: A Vital Investment: WHO Global Report. 2005. Geneva: World Health Organization. p. 18

2. Bast F, Rani P, Meena D. Chloroplast DNA phylogeography of holy basil (Ocimum tenuiflorum) in Indian subcontinent. ScientificWorldJournal. 2014. Vol. 2014:847–482

3. Singh N, Hoette Y, Miller R. Tulsi: The Mother Medicine of Nature. 2010. 2nd ed. Lucknow: International Institute of Herbal Medicine. p. 28–47

4. Mahajan N, Rawal S, Verma M, Poddar M, Alok S. A phytopharmacological overview on Ocimum species with special emphasis on Ocimum sanctum. Biomed Prev Nutr. 2013. Vol. 3:185–92

5. Mohan L, Amberkar MV, Kumari M. Ocimum sanctum linn. (TULSI)-an overview. Int J Pharm Sci Rev Res. 2011. Vol. 7:51–3

6. Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacogn Rev. 2010. Vol. 4:95–105. [PubMed]

7. Mondal S, Mirdha BR, Mahapatra SC. The science behind sacredness of Tulsi (Ocimum sanctum Linn.). Indian J Physiol Pharmacol. 2009. Vol. 53:291–306. [PubMed]

8. Wangcharoen W, Morasuk W. Antioxidant capacity and phenolic content of holy basil. Songklanakarin J Sci Technol. 2007. Vol. 29:1407–15

9. Panda VS, Naik SR. Evaluation of cardioprotective activity of Ginkgo biloba and Ocimum sanctum in rodents. Altern Med Rev. 2009. Vol. 14:161–71. [PubMed]

10. Shivananjappa M, Joshi M. Aqueous extract of tulsi (Ocimum sanctum) enhances endogenous antioxidant defenses of human hepatoma cell line (HepG2). J Herbs Spices Med Plants. 2012. Vol. 18:331–48

11. Manikandan P, Murugan RS, Abbas H, Abraham SK, Nagini S. Ocimum sanctum Linn. (Holy Basil) ethanolic leaf extract protects against 7,12-dimethylbenz (a) anthracene-induced genotoxicity, oxidative stress, and imbalance in xenobiotic-metabolizing enzymes. J Med Food. 2007. Vol. 10:495–502. [PubMed]

12. Siddique YH, Ara G, Beg T, Afzal M. Anti-genotoxic effect of Ocimum sanctum L. extract against cyproterone acetate induced genotoxic damage in cultured mammalian cells. Acta Biol Hung. 2007. Vol. 58:397–409. [PubMed]

13. Jha AK, Jha M, Kaur J. Ethanolic extracts of Ocimum sanctum, Azadirachta indica and Withania somnifera cause apoptosis in SiHa cells. Res J Pharm Biol Chem. 2012. Vol. 3:557–62

14. Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model. Singapore Med J. 2008. Vol. 49:814–22. [PubMed]

15. Rastogi S, Shukla Y, Paul BN, Chowdhuri DK, Khanna SK, Das M. Protective effect of Ocimum sanctum on 3-methylcholanthrene, 7,12-dimethylbenz (a) anthracene and aflatoxin B1 induced skin tumorigenesis in mice. Toxicol Appl Pharmacol. 2007. Vol. 224:228–40. [PubMed]

16. Shah K, Verma RJ. Protection against butyl p-hydroxybenzoic acid induced oxidative stress by Ocimum sanctum extract in mice liver. Acta Pol Pharm. 2012. Vol. 69:865–70. [PubMed]

17. Enayatallah SA, Shah SN, Bodhankar SL. A study of hepatoprotective activity of Ocimum sanctum (Krishna tulas) extracts in chemically induced liver damage in albino mice. J Ecophysiol Occup Health. 2004. Vol. 4:89–96

18. Shyamala AC, Devaki T. Studies on peroxidation in rats ingesting copper sulphate and effect of subsequent treatment with Ocimum sanctum. J Clin Biochem Nutr. 1996. Vol. 20:113–9

19. Bawankule DU, Pal A, Gupta S, Yadav S, Misra A, Rastogi S, et al.. Protective effect of Ocimum sanctum on ethanol-induced oxidative stress in Swiss Albino Mice brain. Toxicol Int. 2008. Vol. 5:121–5

20. Verma P, Kedia DK, Nath A. Protective effect of Ocimum sanctum leaf extracts against rogor induced ovarian toxicity in Clarias batrachus Linn. J Ecophysiology Occup Health. 2007. Vol. 7:177–84

21. Khanna A, Shukla P, Tabassum S. Role of Ocimum sanctum as a genoprotective agent on chlorpyrifos-induced genotoxicity. Toxicol Int. 2011. Vol. 18:9–13. [PubMed]

22. Bharath BK, Anjaneyulu Y, Srilatha C. Imuuno-modulatory effect of Ocimum sanctum against endosulfan induced immunotoxicity. Vet World. 2011. Vol. 4:25–7

23. Mediratta PK, Tanwar K, Reeta KH, Mathur R, Benerjee BD, Singh S, et al.. Attenuation of the effect of lindane on immune responses and oxidative stress by Ocimum sanctum seed oil (OSSO) in rats. Indian J Physiol Pharmacol. 2008. Vol. 52:171–7. [PubMed]

24. Makwana M, Rathore HS. Prevention of hepatorenal toxicity of acetaminophen with Ocimum sanctum in mice. Int J Pharm Technol. 2011. Vol. 3:1385–96

25. Mahaprabhu R, Bhandarkar AG, Jangir BL, Rahangadale SP, Kurkure NV. Ameliorative effect of Ocimum Sanctum on meloxicam induced toxicity in wistar rats. Toxicol Int. 2011. Vol. 18:130–6. [PubMed]

26. Lahon K, Das S. Hepatoprotective activity of Ocimum sanctum alcoholic leaf extract against paracetamol-induced liver damage in Albino rats. Pharmacognosy Res. 2011. Vol. 3:13–8. [PubMed]

27. Pemminati S, Nair V, Dorababu P, Gopalakrishna HN, Pai MR. Effect of ethanolic leaf extract of Ocimum sanctum on haloperidol-induced catalepsy in albino mice. Indian J Pharmacol. 2007. Vol. 39:87–9

28. Ubaid RS, Anantrao KM, Jaju JB, Mateenuddin M. Effect of Ocimum sanctum (OS) leaf extract on hepatotoxicity induced by antitubercular drugs in rats. Indian J Physiol Pharmacol. 2003. Vol. 47:465–70. [PubMed]

29. Karamala SK, Srilatha C, Anjaneyulu Y, ChandraSekharaRao TS, Sreenivasulu D, Pidugu AP. Hematobiochemical changes of lead poisoning and amelioration with Ocimum sanctum in wistar albino rats. Vet World. 2011. Vol. 4:260–3

30. Sharmila Banu G, Kumar G, Murugesan AG. Effects of leaves extract of Ocimum sanctum L. on arsenic-induced toxicity in Wistar albino rats. Food Chem Toxicol. 2009. Vol. 47:490–5. [PubMed]

31. Sharma MK, Kumar M, Kumar A. Ocimum sanctum aqueous leaf extract provides protection against mercury induced toxicity in Swiss albino mice. Indian J Exp Biol. 2002. Vol. 40:1079–82. [PubMed]

32. Bhartiya US, Raut YS, Joseph LJ. Protective effect of Ocimum sanctum L after high-dose 131iodine exposure in mice: An in vivo study. Indian J Exp Biol. 2006. Vol. 44:647–52. [PubMed]

33. Joseph LJ, Bhartiya US, Raut YS, Hawaldar RW, Nayak Y, Pawar YP, et al.. Radioprotective effect of ocimum sanctum and amifostine on the salivary gland of rats after therapeutic radioiodine exposure. Cancer Biother Radiopharm. 2011. Vol. 26:737–43. [PubMed]

34. Reshma K, Kamalaksh S, Bindu YS, Pramod K, Asfa A, Amritha D, et al.. Tulasi (Ocimum Sanctum) as radioprotector in head and neck cancer patients undergoing radiation therapy. Biomedicine. 2012. Vol. 32:39–44

35. Singh N, Verma P, Pandey BR, Bhalla M. Therapeutic Potential of Ocimum sanctum in prevention and treatment of cancer and exposure to radiation: An overview. Int J Pharm Sci Drug Res. 2012. Vol. 4:97–104

36. Uma Devi P, Ganasoundari A, Vrinda B, Srinivasan KK, Unnikrishnan MK. Radiation protection by the Ocimum flavonoids orientin and vicenin: Mechanisms of action. Radiat Res. 2000. Vol. 154:455–60. [PubMed]

37. Reshma K, Rao AV, Dinesh M, Vasudevan DM. Radioprotective effects of ocimum flavonoids on leukocyte oxidants and antioxidants in oral cancer. Indian J Clin Biochem. 2008. Vol. 23:171–5. [PubMed]

38. Bhartiya US, Joseph LJ, Raut YS, Rao BS. Effect of Ocimum sanctum, turmeric extract and vitamin E supplementation on the salivary gland and bone marrow of radioiodine exposed mice. Indian J Exp Biol. 2010. Vol. 48:566–71. [PubMed]

39. Monga J, Sharma M, Tailor N, Ganesh N. Antimelanoma and radioprotective activity of alcoholic aqueous extract of different species of Ocimum in C (57) BL mice. Pharm Biol. 2011. Vol. 49:428–36. [PubMed]

40. Uma Devi P, Ganasoundari A, Rao BS, Srinivasan KK. In vivo radioprotection by ocimum flavonoids: Survival of mice. Radiat Res. 1999. Vol. 151:74–8. [PubMed]

41. Nayak V, Devi PU. Protection of mouse bone marrow against radiation-induced chromosome damage and stem cell death by the ocimum flavonoids orientin and vicenin. Radiat Res. 2005. Vol. 163:165–71. [PubMed]

42. Samson J, Sheeladevi R, Ravindran R. Oxidative stress in brain and antioxidant activity of Ocimum sanctum in noise exposure. Neurotoxicology. 2007. Vol. 28:679–85. [PubMed]

43. Archana R, Namasivayam A. A comparative study of different crude extracts of Ocimum sanctum on noise stress. Phytother Res. 2002. Vol. 16:579–80. [PubMed]

44. Sembulingam K, Sembulingam P, Namasivayam A. Effect of ocimum sanctum linn on changes in leucocytes of albino rats induced by acute noise stress. Indian J Physiol Pharmacol. 1999. Vol. 43:137–140

45. Sembulingam K, Sembulingam P, Namasivayam A. Effect of Ocimum sanctum Linn on the changes in central cholinergic system induced by acute noise stress. J Ethnopharmacol. 2005. Vol. 96:477–82. [PubMed]

46. Ahmad MZ, Ali M, Mir SR. Anti-diabetic activity of Ocimum sanctum L. roots and isolation of new phytoconstituents using two-dimensional nuclear magnetic resonance spectroscopy. J Pharmacogn Phytother. 2012. Vol. 4:75–85

47. Singh PK, Baxi D, Banerjee S, Ramachandran AV. Therapy with methanolic extract of Pterocarpus marsupium Roxb and Ocimum sanctum Linn reverses dyslipidemia and oxidative stress in alloxan induced type I diabetic rat model. Exp Toxicol Pathol. 2012. Vol. 64:441–8. [PubMed]

48. Suanarunsawat T, Songsak T. Anti-hyperglycaemic and anti-dyslipidaemic effect of dietary supplement of white Ocimum Sanctum Linnean before and after STZ-induced diabetes mellitus. Int J Diabetes Metab. 2005. Vol. 13:18–23

49. Suanarunsawat T, Ayutthaya WD, Songsak T, Thirawarapan S, Poungshompoo S. Lipid-lowering and antioxidative activities of aqueous extracts of Ocimum sanctum L. leaves in rats fed with a high-cholesterol diet. Oxid Med Cell Longev. 2011. Vol. 2011:962025[PubMed]

50. Dahiya K, Sethi J, Dhankhar R, Singh V, Singh SB, Yadav M, et al.. Effect of Ocimum sanctum on homocysteine levels and lipid profile in healthy rabbits. Arch Physiol Biochem. 2011. Vol. 117:8–11. [PubMed]

51. Reddy SS, Karuna R, Baskar R, Saralakumari D. Prevention of insulin resistance by ingesting aqueous extract of Ocimum sanctum to fructose-fed rats. Horm Metab Res. 2008. Vol. 40:44–9. [PubMed]

52. Suanarunsawat T, Ayutthaya WD, Songsak T, Rattanamahaphoom J. Anti-lipidemic actions of essential oil extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet. J Appl Biomed. 2009. Vol. 7:45–53

53. Samak G, Rao MS, Kedlaya R, Vasudevan DM. Hypolipidemic efficacy of Ocimum sanctum in the prevention of atherogenesis in male albino rabbits. Pharmacologyonline. 2007. Vol. 2:115–27

54. Agrawal P, Rai V, Singh RB. Randomized placebo-controlled, single blind trial of holy basil leaves in patients with noninsulin-dependent diabetes mellitus. Int J Clin Pharmacol Ther. 1996. Vol. 34:406–9. [PubMed]

55. Rai V, Mani UV, Iyer UM. Effect of Ocimum sanctum leaf powder on blood lipoproteins, glycated proteins and total amino acids in patients with non-insulin-dependent diabetes mellitus. J Nutr Environ Med. 1997. Vol. 7:113–8

56. Devra DK, Mathur KC, Agrawal RP, Bhadu I, Goyal S, Agarwal V. Effect of tulsi (Ocimum sanctum Linn) on clinical and biochemical parameters of metabolic syndrome. J Nat Remedies. 2012. Vol. 12:63–7

57. Kochhar A, Sharma N, Sachdeva R. Effect of supplementation of Tulsi (Ocimum sanctum) and Neem (Azadirachta indica) leaf powder on diabetic symptoms, anthropometric parameters and blood pressure of non insulin dependent male diabetics. Ethno-Med. 2009. Vol. 3:5–9

58. Dusane MB, Joshi BN. Islet protective and insulin secretion property of Murraya koenigii and Ocimum tenuflorum in streptozotocin-induced diabetic mice. Can J Physiol Pharmacol. 2012. Vol. 90:371–8. [PubMed]

59. Hannan JM, Marenah L, Ali L, Rokeya B, Flatt PR, Abdel-Wahab YH. Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic beta-cells. J Endocrinol. 2006. Vol. 189:127–36. [PubMed]

60. Chattopadhyay RR. Hypoglycemic effect of Ocimum sanctum leaf extract in normal and streptozotocin diabetic rats. Indian J Exp Biol. 1993. Vol. 31:891–3. [PubMed]

61. Gholap S, Kar A. Hypoglycaemic effects of some plant extracts are possibly mediated through inhibition in corticosteroid concentration. Pharmazie. 2004. Vol. 59:876–8. [PubMed]

62. Kothari A, Sharma S. Evaluation of anti-inflammatory effect of fresh tulsi leaves (Ocimum Sanctum) against different mediators of inflammation in albino rats. Int J Pharm Sci Rev Res. 2012. Vol. 14:119–23

63. Fernández PB, Figueredo YN, Dominguez CC, Hernández IC, Sanabria MLG, González R, et al.. Anti-inflammatory effect of lyophilized aqueous extract of Ocinum tenuiflorum on rats. Acta Farm Bonaerense. 2004. Vol. 23:92–7

64. Thakur K, Pitre KS. Anti-inflammatory activity of extracted eugenol from Ocimum sanctum L. leaves. Rasayan J Chem. 2009. Vol. 2:472–4

65. Singh S, Majumdar DK. Effect of Ocimum sanctum fixed oil on vascular permeability and leucocytes migration. Indian J Exp Biol. 1999. Vol. 37:1136–8. [PubMed]

66. Singh S, Majumdar DK. Evaluation of antiinflammatory activity of fatty acids of Ocimum sanctum fixed oil. Indian J Exp Biol. 1997. Vol. 35:380–3. [PubMed]

67. Singh S. Comparative evaluation of antiinflammatory potential of fixed oil of different species of Ocimum and its possible mechanism of action. Indian J Exp Biol. 1998. Vol. 36:1028–31. [PubMed]

68. Singh S, Majumdar DK. Anti-inflammatory and antipyretic activities of Ocimum sanctum fixed oil. Int Pharmacogn. 1995. Vol. 33:288–92

69. Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine. 2000. Vol. 7:7–13. [PubMed]

70. Kalabharathi HL, Suresha RN, Pragathi B, Pushpa VH, Satish AM. Anti inflammatory activity of fresh tulsi leaves (Ocimum Sanctum) in albino rats. International Journal of Pharma and Bio Sciences. 2011. Vol. 2:45–50

71. Vasudevan P, Kashyap S, Sharma S. Bioactive botanicals from basil (Ocimum sp.). J Sci Ind Res (C). 1999. Vol. 58:332–8

72. Mediratta PK, Sharma KK, Singh S. Evaluation of immunomodulatory potential of Ocimum sanctum seed oil and its possible mechanism of action. J Ethnopharmacol. 2002. Vol. 80:15–20. [PubMed]

73. Hemalatha R, Babu KN, Karthik M, Ramesh R, Kumar BD, Kumar PU. Immunomodulatory activity and Th1/Th2 cytokine response of Ocimum sanctum in myelosuppressed swiss albino mice. Trends Med Res. 2011. Vol. 6:23–31

74. Tripathi AK, Rajora VS, Gupta DK, Shukla SK. Immunomodulatory activity of Ocimum sanctum and its influence on cyclophosphamide induced immunosupression. Indian J Anim Sci. 2008. Vol. 78:33–6

75. Goel A, Singh DK, Kumar S, Bhatia AK. Immunomodulating property of Ocimum sanctum by regulating the IL-2 production and its mRNA expression using rat's splenocytes. Asian Pac J Trop Med. 2010. Vol. 3:8–12

76. Pavaraj M, Balasubramanian V, Baskaran S, Ramasamy P. Development of immunity by extract of medicinal plant Ocimum sanctum on common carp Cyprinus carpio (L.). Res J Immunol. 2011. Vol. 4:12–8

77. Chitra G, Krishnaveni N. Immunostimulatory effect of Ocimum sanctum leaf extract on the Indian major carp, Catla catla. Plant Archives. 2011. Vol. 11:213–4

78. Mondal S, Varma S, Bamola VD, Naik SN, Mirdha BR, Padhi MM, et al.. Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum Linn.) leaf extract on healthy volunteers. J Ethnopharmacol. 2011. Vol. 136:452–6. [PubMed]

79. Ali H, Dixit S. In vitro antimicrobial activity of flavanoids of Ocimum sanctum with synergistic effect of their combined form. Asian Pac J Trop Dis. 2012. Vol. 2:S396–8

80. Singh S, Malhotra M, Majumdar DK. Antibacterial activity of Ocimum sanctum L. fixed oil. Indian J Exp Biol. 2005. Vol. 43:835–7. [PubMed]

81. Mandal S, Mandal MD, Pal NK. Enhancing chloramphenicol and trimethoprim in vitro activity by Ocimum sanctum Linn.(Lamiaceae) leaf extract against Salmonella enterica serovar Typhi. Asian Pac J Trop Med. 2012. Vol. 5:220–4. [PubMed]

82. Parag S, Vijyayshree N, Rami B, Patil B. Antibacterial activity of Ocimum sanctum Linn. and its application in water purification. Res J Chem Environ. 2010. Vol. 14:46–50

83. Farivar TN, Fard AH, Zahedani SS, Naderi M, Moud BS. Anti tuberculosis effect of Ocimum sanctum extracts in in vitro and macrophage culture. J Med Sci. 2006. Vol. 6:348–51

84. Shokeen P, Ray K, Bala M, Tandon V. Preliminary studies on activity of Ocimum sanctum, Drynaria quercifolia, and Annona squamosa against Neisseria gonorrhoeae. Sex Transm Dis. 2005. Vol. 32:106–11. [PubMed]

85. Sawarkar HA, Khadabadi SS, Mankar DM, Farooqui IA, Jagtap NS. Development and biological evaluation of herbal anti-acne gel. Int J PharmTech Res. 2010. Vol. 2:2028–31

86. Yucharoen R, Anuchapreeda S, Tragoolpua Y. Anti-herpes simplex virus activity of extracts from the culinary herbs Ocimum sanctum L., Ocimum basilicum L. and Ocimum americanum L. Afri J Biotechnol. 2011. Vol. 10:860–6

87. Suzuki A, Shirota O, Mori K, Sekita S, Fuchino H, Takano A, et al.. Leishmanicidal active constituents from Nepalese medicinal plant Tulsi (Ocimum sanctum L.). Chem Pharm Bull (Tokyo). 2009. Vol. 57:245–51. [PubMed]

88. Saini A, Sharma S, Chhibber S. Induction of resistance to respiratory tract infection with Klebsiella pneumoniae in mice fed on a diet supplemented with tulsi (Ocimum sanctum) and clove (Syzgium aromaticum) oils. J Microbiol Immunol Infect. 2009. Vol. 42:107–13. [PubMed]

89. Deo SS, Inam F, Mahashabde RP. Antimicrobial activity and HPLC fingerprinting of crude ocimum extracts. E-Journal of Chemistry. 2011. Vol. 8:1430–7

90. Balakumar S, Rajan S, Thirunalasundari T, Jeeva S. Antifungal activity of Ocimum sanctum Linn.(Lamiaceae) on clinically isolated dermatophytic fungi. Asian Pac J Trop Med. 2011. Vol. 4:654–7. [PubMed]

91. Das J, Buragohain B, Srivastava RB. In vitro evaluation of ocimum sanctum leaf extract against dermatophytes and opportunistic fungi. Asian J Microbiol Biotechnol Environ Sci. 2010. Vol. 12:789–92

92. Chandra R, Dwivedi V, Shivam K, Jha AK. Detection of antimicrobial activity of Oscimum sanctum (Tulsi) and trigonella foenum graecum (Methi) against some selected bacterial and fungal strains. Res J Pharm Biol Chem Sci. 2011. Vol. 2:809–13

93. Rajamma AJ, Dubey S, Sateesha SB, Tiwari SN, Ghosh SK. Comparative larvicidal activity of different species of Ocimum against Culex Quinquefasciatus. Nat Prod Res. 2011. Vol. 25:1916–22. [PubMed]

94. Gbolade AA, Lockwood GB. Toxicity of Ocimum sanctum L. essential oil to Aedes aegypti Larvae and its chemical composition. J Essent Oil-Bear Plants. 2008. Vol. 11:148–53

95. Inbaneson SJ, Sundaram R, Suganthi P. In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant Ocimum species against Plasmodium falciparum. Asian Pac J Trop Med. 2012. Vol. 5:103–6. [PubMed]

96. Kumar S, Gupta HP, Prasad S, Rajora VS, Prasad JK. Antibacterial properties of garlic and tulsi in repeat breeding crossbred cows. Indian Vet J. 2011. Vol. 88:28–30

97. Sadekar RO, Pimprikar NM, Bhandarkar AG, Barmase BS. Immunomodulating effect of Ocimum sanctum linn. Dry leaf powder on humoral immune response in poultry naturally infected with IBD virus. Indian Vet J. 1998. Vol. 75:73–4

98. Deka RS. Influence of tulsi (Ocimum sanctum) and ashwagandha (Withania somnifera) supplementation on production of organic meat in goats. Veterinary Practitioner. 2009. Vol. 10:57–9

99. Bora DS, Khanikor B. Selective toxicity of Ageratum conyzoides and Ocimum sanctum against Exorista sorbillans (Diptera: Tachinidae) and Antheraea ussama (Lepidoptera: Saturniidae). Natl Acad Sci Lett. 2011. Vol. 34:9–14

100. Mishra P, Mishra S. Study of antibacterial activity of ocimum sanctum extract against gram positive and gram negative bacteria. Am J Food Technol. 2011. Vol. 6:336–41

101. Kumar A, Shukla R, Singh P, Dubey NK. Chemical composition, antifungal and antiaflatoxigenic activities of Ocimum sanctum L. essential oil and its safety assessment as plant based antimicrobial. Food Chem Toxicol. 2010. Vol. 48:539–43. [PubMed]

102. Upadhyaya S, Behera J, Tewari SN. Integrated management of foliar blast through ecofriendly formulated product, Oscext-e developed from Ocimum sanctum ethanolic extract. Arch Phytopathology Plant Protect. 2012. Vol. 45:2290–300

103. Kumar A, Dubey NK, Srivastava S. Antifungal evaluation of Ocimum sanctum essential oil against fungal deterioration of raw materials of Rauvolfia serpentina during storage. Ind Crops Prod. 2013. Vol. 45:30–5

104. Wani NS, Bhalerao AK, Ranaware VP, Zanje R. Formulation and evaluation of herbal sanitizer. Int J PharmTech Res. 2013. Vol. 5:40–3

105. Kukreja BJ, Dodwad V. Herbal mouthwashes-A gift of nature. Int J Pharma Bio Sci. 2012. Vol. 3:46–52

106. Malik K, Arora G, Singh I. Ocimum sanctum seeds, a natural superdisintegrant: Formulation and evaluation of fast melt tablets of nimesulide. Polim Med. 2012. Vol. 42:49–59. [PubMed]

107. Agarwal P, Nagesh L. Comparative evaluation of efficacy of 0.2% Chlorhexidine, Listerine and Tulsi extract mouth rinses on salivary Streptococcus mutans count of high school children--RCT. Contemp Clin Trials. 2011. Vol. 32:802–8. [PubMed]

108. Malhotra R, Grover V, Kapoor A, Saxena D. Comparison of the effectiveness of a commercially available herbal mouthrinse with chlorhexidine gluconate at the clinical and patient level. J Indian Soc Periodontol. 2011. Vol. 15:349–52. [PubMed]

109. Singh S, Taneja M, Majumdar DK. Biological activities of Ocimum sanctum L. fixed oil--an overview. Indian J Exp Biol. 2007. Vol. 45:403–12. [PubMed]

110. Shetty S, Udupa S, Udupa L. Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evid Based Complement Alternat Med. 2008. Vol. 5:95–101. [PubMed]

111. Goel A, Kumar S, Singh DK, Bhatia AK. Wound healing potential of Ocimum sanctum Linn. with induction of tumor necrosis factor-alpha. Indian J Exp Biol. 2010. Vol. 48:402–6. [PubMed]

112. Kath RK, Gupta RK. Antioxidant activity of hydroalcoholic leaf extract of ocimum sanctum in animal models of peptic ulcer. Indian J Physiol Pharmacol. 2006. Vol. 50:391–6. [PubMed]

113. Singh S, Majumdar DK. Evaluation of the gastric antiulcer activity of fixed oil of Ocimum sanctum (Holy Basil). J Ethnopharmacol. 1999. Vol. 65:13–9. [PubMed]

114. Dharmani P, Kuchibhotla VK, Maurya R, Srivastava S, Sharma S, Palit G. Evaluation of anti-ulcerogenic and ulcer-healing properties of Ocimum sanctum Linn. J Ethnopharmacol. 2004. Vol. 93:197–206. [PubMed]

115. Goel RK, Sairam K, Dorababu M, Prabha T, Rao CV. Effect of standardized extract of Ocimum sanctum Linn. on gastric mucosal offensive and defensive factors. Indian J Exp Biol. 2005. Vol. 43:715–21. [PubMed]

116. Chatterjee M, Verma P, Maurya R, Palit G. Evaluation of ethanol leaf extract of Ocimum sanctum in experimental models of anxiety and depression. Pharm Biol. 2011. Vol. 49:477–83. [PubMed]

117. Tabassum I, Siddiqui ZN, Rizvi SJ. Effects of Ocimum sanctum and Camellia sinensis on stress-induced anxiety and depression in male albino Rattus norvegicus. Indian J Pharmacol. 2010. Vol. 42:283–8. [PubMed]

118. Raghavendra M, Maiti R, Kumar S, Acharya SB. Role of Ocimum sanctum in the experimental model of Alzheimer's disease in rats. Int J Green Pharm. 2009. Vol. 3:6–15

119. Pemminati S, Gopalakrishna HN, Venkatesh V, Rai A, Shetty S, Vinod A, et al.. Anxiolytic effect of acute administration of ursolic acid in rats. Res J Pharm Biol Chem Sci. 2011. Vol. 2:431–7

120. Moinuddin G, Devi K, Satish H, Khajuria DK. Comparative pharmacological evaluation of Ocimum sanctum and imipramine for antidepressant activity. Lat Am J Pharm. 2011. Vol. 30:435–9

121. Giridharan VV, Thandavarayan RA, Mani V, Ashok Dundapa T, Watanabe K, Konishi T. Ocimum sanctum Linn. leaf extracts inhibit acetylcholinesterase and improve cognition in rats with experimentally induced dementia. J Med Food. 2011. Vol. 14:912–9. [PubMed]

122. Dokania M, Kishore K, Sharma PK. Effect of Ocimum sanctum extract on sodium nitrite-induced experimental amnesia in mice. Thai J Pharma Sci. 2011. Vol. 35:123–30

123. Joshi H, Parle M. Cholinergic basis of memory improving effect of Ocimum tenuiflorum Linn. Indian J Pharm Sci. 2006. Vol. 68:364–5

124. Bhattacharyya D, Sur TK, Jana U, Debnath PK. Controlled programmed trial of Ocimum sanctum leaf on generalized anxiety disorders. Nepal Med Coll J. 2008. Vol. 10:176–9. [PubMed]

125. Saxena RC, Singh R, Kumar P, Negi MP, Saxena VS, Geetharani P, et al.. Efficacy of an extract of ocimum tenuiflorum (OciBest) in the management of general stress: A double-blind, placebo-controlled study. Evid Based Complement Alternat Med. 2012. Vol. 2012:894509[PubMed]

126. Shukla ST, Kulkarni VH, Habbu PV, Jagadeesh KS, Patil BS, Smita DM. Hepatoprotective and antioxidant activities of crude fractions of endophytic fungi of Ocimum sanctum Linn. in rats. Orient Pharm Exp Med. 2012. Vol. 12:81–91

127. Mishra M. Tulsi to Save Taj Mahal from Pollution Effects. The Times of India, Bennett Coleman and Co. Ltd. 2008.

128. Maller C, Townsend M, St Leger L, Henerson-Wilson C, Pryor A, Prosser L, et al.. Healthy parks, healthy people: The health benefits of contact with nature in a park context. Soc Dev. 2009. Vol. 26:51–83

129. Charles C, Louv R, Bodner L, Guns B. Children and Nature 2008. A Report on the Movement to Reconnect Children to the Natural World. 2008. Santa Fe: Children and Nature Network. p. 9–11

130. Singh P, Mittal VK, Gupta SC. Trace elements in typical herbs as an indicator of environmental pollution. Indian J Environ Prot. 2003. Vol. 23:1114–9

131. Jürges G, Beyerle K, Tossenberger M, Häser A, Nick P. Development and validation of microscopical diagnostics for ‘Tulsi’ (Ocimum tenuiflorum L.) in ayurvedic preparations. Eur Food Res Technol. 2009. Vol. 229:99–106

132. Chanda D, Pal A, Shanker K. Application of HPLC fingerprints for defining in vivo safety profile of Tulsi (Ocimum Sanctum). Medicinal Chemistry Research. 2013. Vol. 22:219–24

133. United Nations Conference on Trade and Development. Trade and Environment Review 2013. In: 2013. Wake Up Before It Is Too Late: Make Agriculture Truly Sustainable Now for Food Security in a Changing Climate; Geneva, United Nations. p. 1–7