+1 Recommend
    • Review: found
    Is Open Access

    Review of 'The Spherical Nucleic Acids mRNA Detection Paradox'

    The Spherical Nucleic Acids mRNA Detection ParadoxCrossref
    An important re-evaluation of SmartFlares as detectors of cytoplasmic mRNA
    Average rating:
        Rated 4.5 of 5.
    Level of importance:
        Rated 5 of 5.
    Level of validity:
        Rated 4 of 5.
    Level of completeness:
        Rated 5 of 5.
    Level of comprehensibility:
        Rated 4 of 5.
    Competing interests:

    Reviewed article

    • Record: found
    • Abstract: found
    • Article: found
    Is Open Access

    The Spherical Nucleic Acids mRNA Detection Paradox

    From the 1950s onwards, our understanding of the formation and intracellular trafficking of membrane vesicles was informed by experiments in which cells were exposed to gold nanoparticles and their uptake and localisation, studied by electron microscopy. In the last decade, building on progress in the synthesis of gold nanoparticles and their controlled functionalisation with a large variety of biomolecules (DNA, peptides, polysaccharides), new applications have been proposed, including the imaging and sensing of intracellular events. Yet, as already demonstrated in the 1950s, uptake of nanoparticles results in confinement within an intracellular vesicle which in principle should preclude sensing of cytosolic events. To study this apparent paradox, we focus on a commercially available nanoparticle probe that detects mRNA through the release of a fluorescently-labelled oligonucleotide (unquenching the fluorescence) in the presence of the target mRNA. Using electron, fluorescence and photothermal microscopy, we show that the probes remain in endocytic compartments and that they do not report on mRNA level. We suggest that the validation of any nanoparticle-based probes for intracellular sensing should include a quantitative and thorough demonstration that the probes can reach the cytosolic compartment.

      Review information

      This work has been published open access under Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Conditions, terms of use and publishing policy can be found at www.scienceopen.com.

      Review text

      This article by Lévy et al aims to debunk the claims that SmartFlares can detect mRNA in the cytoplasm of cells. The authors briefly summarise the current state of the literature accurately: there is no cell biological reason for thinking that these nanoparticles should actually work. Good evidence for endosomal escape has not been documented so far. They then set out to characterise SmartFlare activity and conclude that these particles do not work as advertised. This is an important, but thankless, task. Important because it will prevent others from wasting time and resources on something that doesn't work. Thankless because it can often take a great deal of effort to overturn (attractive) ideas.

      As a cell biologist, one area for improvement in this paper that I see would be to quantify more cells that have been doubly labelled to better characterise the compartment that the SmartFlares end up in. Figure 4 shows these experiments, but we only see one or two cells, with a single Mander's coefficient. I would prefer to see more cells quantified. Maybe the location of the vesicles containing SmartFlares is variable, but this could be better documented than it is now. Related to this:

      "We labelled the endocytic pathway using a fluorescently-labelled 10 kDa dextran, both to show that constitutive uptake was occurring in our system, but also to label all of the compartments of the pathway. As expected, the dextran labelled every cell in the field with homogeneous intensity puncta (Figure 4B). There were approximately the same number of vesicles per cell when corrected for cell size. Interestingly, the dextran and SmartFlares rarely labelled the same compartments (Figure 4C) even at a 2 hour time point (Supplementary Figure 1).

      As the dextran may be excluded from receptor-mediated endosomes (by unknown mechanisms) we also used immunofluorescence to label the recycling (transferrin-receptor positive) and terminal
      (lysosomal) compartments of the endocytic pathway (Figure 4D-F and G-I respectively). Once again, the SmartFlares showed little overlap with either of these compartments (Figure 4C,F,I, Manders’ coefficients inset), suggesting a parallel but largely non-overlapping compartment."

      I don't agree with this interpretation. From the examples shown it looks like there is significant colocalisation with fluorescent dextran (Fig 4C and Supp Fig 1). This condition also has the highest Manders coefficient, compared to Tf and LAMP1 staining. Where there is also some overlap, albeit limited.

      It seems clear from the work presented in earlier figures that the particles are retained in vesicles. There's partial overlap with these compartments at the resolution of light microscopy. Some more quantification would be good to try to nail down where the SmartFlares end up. 

      I thought to improve presentation that the figures could do with more labelling and less lettering. For example on Fig 4, the columns could be labelled SmartFlares, Stain and Merge (l-r). The rows could be labelled with 10K dextran, TfR and LAMP-1 (top to bottom). This means that the figure can be understood by just looking at it. 

      There is no label on the y-axis in Fig 3E.

      Very minor
      1. I'm not a big fan of using number of Web of Science search results as an argument (Introduction). The number of papers on Gold Nanoparticles may be increasing since 2007, but then so are the number of papers on anything. It needs to be normalised to be meaningful. It's also a shame that only 5 papers have cited Harford et al., but it's an old paper, maybe people are citing reviews that cover this paper instead?
      2. Technai is spelled Tecnai
      3. Extra I J K below the panels in Fig 4 need to be removed.


      We would like to thank Steve Royal for his review, comments and helpful critique. Version 2 has been submitted and is currently in the type-setting stage.

      Upon re-reading, we accept that the text of version 1 was not clear about the number of cells quantified for the double labelled experiments. The single Manders' coefficient per condition provided in Figure 4 is the mean Manders' coefficient from 12 fields (TFN-R & LAMP1) and 24 fields (dextran). This may also go towards explaining why the colocalisation in the example images may seem disparate with the values. We have clarified these numbers in the text, Figure legends and Methods.

      We have altered the text to less subjectively describe the colocalisation of the SmartFlares, removing the word 'rarely' which does not accurately describe a Manders' colocalisation coefficient of 0.43.

      While we would like to perform an exhaustive and overlapping study on the intracellular localisation of SmartFlares, we feel that it is beyond the the scope of this paper which principally presents a lack of endosomal escape. We point interested parties to an excellent paper by the Mirkin group (doi:10.1021/ja503010a) looking at the intracellular fate of SNAs.

      We have taken the suggestion under advisement and added more labels to the figures. We hope that this clarifies the paper for readers.

      An axis label has been added to the graph in Figure 3E.

      After a significant amount of investigation, the senior author of the paper has managed to find what he believes to be some of the first papers which allude to endosomal escape of gold nanoparticles. We have adjusted the text of the introduction to this effect removing the need to cite Web of Science. More details can also be found at the following link: [http://web.archive.org/web/20160222142449/https://raphazlab.wordpress.com/2016/02/01/nanoparticles-cell-membranes-history-of-a-science-fiction/]

      The spelling of Tecnai has been corrected.

      2016-03-15 15:18 UTC

      Comment on this review