2015-05-07
Average rating: | Rated 4 of 5. |
Level of importance: | Rated 4 of 5. |
Level of validity: | Rated 4 of 5. |
Level of completeness: | Rated 4 of 5. |
Level of comprehensibility: | Rated 4 of 5. |
Competing interests: | None |
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ScienceOpen disciplines: | Life sciences |
Keywords: | Seed sprouts, Fluorescence In Situ Hybridization (FISH), Rapid detection, Flow Cytometry, Salmonella |
Bisha and Brehm-Stecher reported the combined DNA-based fluorescence in situ hybridization (FISH) with flow cytometry (FCM) method to detect Salmonella in seed sprouts samples. Quick and sensitive method to detect Salmonella is important in terms of food safety concern, this manuscript is of scientific merit and I recommend to be acceptable for publication. However, I do have some suggestions:
The authors put too many introductory materials in the Discussion section. The first 5 paragraphs of the Discussion should be removed to the Introduction section or omit some of them. They should discuss the results presented in the manuscript more.
P2, Bacterial strains and culture conditions: spell out Escherichia coli ATCC 25922
P4, Pre-analytical sample preparation: Individual samples (1.3 ml) were aliquoted into in 1.5 ml polypropylene microcentrifuge tubes.., please remove “in”.