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    Review of 'DNA Double-Strand Break Repairs and Their Application in Plant DNA Integration'

    DNA Double-Strand Break Repairs and Their Application in Plant DNA IntegrationCrossref
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    DNA Double-Strand Break Repairs and Their Application in Plant DNA Integration

    Double-strand breaks (DSBs) are considered to be one of the most harmful and mutagenic forms of DNA damage. They are highly toxic if unrepaired, and can cause genome rearrangements and even cell death. Cells employ two major pathways to repair DSBs: homologous recombination (HR) and non-homologous end-joining (NHEJ). In plants, most applications of genome modification techniques depend on the development of DSB repair pathways, such as Agrobacterium-mediated transformation (AMT) and gene targeting (GT). In this paper, we review the achieved knowledge and recent advances on the DNA DSB response and its main repair pathways; discuss how these pathways affect Agrobacterium-mediated T-DNA integration and gene targeting in plants; and describe promising strategies for producing DSBs artificially, at definite sites in the genome.

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      Does VirD2 directly/indirectly involved in the process of T-DNA integration? How does VirD2 participate in the integration process?

      How about T-circle? T-circles are double-strand circular moleculars that contains T-DNA that is near T-DNA RB and LB, they are formed in plants and normally have a lot of characteristics of T-DNA/plant DNA junctions, are T-circles important/play a role in T-DNA integration?


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