Journal ID (nlm-journal-id): 101186374
Journal ID (pubmed-jr-id): 31761
Journal ID (nlm-ta): Nat Struct Mol Biol
Title:
Nature structural & molecular biology
ISSN
(Print):
1545-9993
ISSN
(Electronic):
1545-9985
Publication date Nihms-submitted: 14
April
2009
Publication date
(Electronic):
8
February
2009
Publication date
(Print):
March
2009
Publication date PMC-release: 12
November
2009
Volume: 16
Issue: 3
Pages: 255-264
Affiliations
[1
]Center for RNA Molecular Biology and Department of Molecular Biology & Microbiology,
Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4960
[2
]Department of Molecular Genetics and Microbiology, State University of New York, Stony
Brook, NY 11794-5222
Author notes
Correspondence should be addressed to J.A.W. (
jaw17@
123456case.edu
) Phone: (216)-368-1876, FAX (216)-368-3055
AUTHOR CONTRIBUTIONS
David McPheeters performed the TRO experiments, designed the real-time PCR assay,
and participated in development of the model as well as writing of the manuscript.
Nicole Cremona constructed and analyzed the chimeric and mutant alleles of
crs1 to define the regulatory element, performed the RNA analyses on
trans-acting factor mutants, qPCR, and expertly proofread the manuscript. Sham Sunder analyzed
processing of
crs1 RNA over a meiotic time course and conducted initial experiments to map the
crs1 regulatory element. Nicole Averbeck mapped the
crs1 RNA termini by RACE and constructed the
crs1 deletion strain. Huei-Mei Chen and Janet Leatherwood discovered the splicing defect
in the
pfs2-11 mutant. Jo Ann Wise wrote the manuscript and contributed to the design and interpretation
of all experiments.
Article
Manuscript ID: nihpa86778
DOI: 10.1038/nsmb.1556
PMC ID: 2776722
PubMed ID: 19198588
SO-VID: 45e329f0-a960-4510-94f9-1da20510033c
Funded by: National Institute of General Medical Sciences : NIGMS
Award ID: R01 GM073217-03
||GM
Funded by: National Institute of General Medical Sciences : NIGMS
Award ID: R01 GM073217-02
||GM
Funded by: National Institute of General Medical Sciences : NIGMS
Award ID: R01 GM073217-01A2
||GM
Funded by: National Institute of General Medical Sciences : NIGMS
Award ID: R01 GM064682-04
||GM
Funded by: National Institute of General Medical Sciences : NIGMS
Award ID: R01 GM038070-15
||GM