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      Isolation and Characterization of Mannanase-Producing Bacteria for Potential Synbiotic Application in Shrimp Farming

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          Abstract

          Prebiotics such as mannan-oligosaccharides (MOS) are a promising approach to improve performance and disease resistance in shrimp. To improve prebiotic utilization, we investigated the potential probiotics and their feasibility of synbiotic use in vitro. Two bacterial isolates, Man26 and Man122, were isolated from shrimp intestines and screened for mannanase, the enzyme for mannan digestion. The crude mannanase from both isolates showed optimal activities at pH 8 with optimum temperatures at 60 °C and 50 °C, respectively. The enzymes remained stable at pH 8–10 for 3 h (>70% relative activity). The thermostability range of Man26 was 20–40 °C for 20 min (>50%), while that of Man122 was 20–60 °C for 30 min (>50%). The Vmax of Man122 against locust bean gum substrate was 41.15 ± 12.33 U·mg−1, six times higher than that of Man26. The Km of Man26 and Man122 were 18.92 ± 4.36 mg·mL−1 and 34.53 ± 14.46 mg·mL−1, respectively. With the addition of crude enzymes, reducing sugars of copra meal, palm kernel cake, and soybean meal were significantly increased (p < 0.05), as well as protein release. The results suggest that Man26 and Man122 could potentially be used in animal feeds and synbiotically with copra meal to improve absorption and utilization of feedstuffs.

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          Most cited references59

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            Colorimetric Method for Determination of Sugars and Related Substances

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              Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

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                Author and article information

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                Journal
                Animals
                Animals
                MDPI AG
                2076-2615
                October 2022
                September 27 2022
                : 12
                : 19
                : 2583
                Article
                10.3390/ani12192583
                84992e91-fc0a-419d-935c-31c6ee6476fb
                © 2022

                https://creativecommons.org/licenses/by/4.0/

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