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      Ameloglyphics: A possible forensic tool for person identification following high temperature and acid exposure

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          Abstract

          Introduction:

          Forensic odontology is a branch that is evolving over time and has opened newer avenues that may help in the identification of individuals. Tooth prints are the enamel rod end patterns on tooth surface and they are considered as a hard tissue analog to fingerprints. Teeth have the highest resistance to most environmental effects like fire, desiccation, and decomposition, and may be used as a forensic evidence.

          Aims and Objectives:

          The aim of the study was to evaluate if the tooth prints could be used for an individual's identification and reproducibility and permanency of these tooth prints after exposing the teeth to acid and various degrees of temperature.

          Materials and Methods:

          90 tooth prints from 20 freshly extracted maxillary premolar teeth were obtained. Cellophane tape technique was used to record enamel rod end patterns on tooth surface. Ten teeth (one from each patient) were immersed in 36.46% hydrochloric acid and the tooth prints were obtained at various intervals (5 min, 10 min, and 20 min). The other 10 teeth (one from each patient) were incinerated and impression was made at various intervals (80° C, 400° C, 600° C, and 750° C). Tooth prints obtained from different teeth (total of 90 tooth prints) were analyzed using Verifinger® standard SDK version 5.0 software.

          Results:

          All the 20 original tooth prints were distinct from each other and no inter-individual or intra-individual similarity was found. The tooth prints from the same tooth after it was exposed to acid or heat were reproducible and showed high to very high similarity with the original tooth print of that particular tooth stored in the database.

          Conclusion:

          Tooth prints may be used as an effective aid in person identification even in adverse conditions such as burn and acid attack injuries.

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          Most cited references11

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          The application of dental methods of identification to human burn victims in a mass disaster.

          This paper deals with the usefulness of dental identification in the case of 28 human burn victims of a bus accident in Spain. Postmortem forensic procedures for identification were used including general external examination, routine photographs and radiographs and complementary biological methods. Dental identification was also used and a description of the method is presented. Dental identification was established in 57% of the cases. When victims were less than 20 years of age, the success rate of identification by dental methods was higher (76% of victims in this age group). The assessment of dental age allowed the establishment of identity of four victims. We recommend that dental procedures be used in human identification after mass disasters. Odontological and radiographic procedures are powerful methods when dealing with burn victims.
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            Development of a quality, high throughput DNA analysis procedure for skeletal samples to assist with the identification of victims from the World Trade Center attacks.

            The attacks on the World Trade Center (WTC) Towers on September 11, 2001, represented the single largest terrorist-related mass fatality incident in the history of the United States. More than 2,700 individuals of varied racial and ethnic background lost their lives that day. Through the efforts of thousands of citizens, including recovery workers, medical examiners, and forensic scientists, the identification of approximately 1,500 victims had been accomplished through June 2003 (the majority of these identifications were made within the first 8-12 months). The principal role of The Bode Technology Group (Bode) in this process was to develop a quality, high throughput DNA extraction and short tandem repeat (STR) analysis procedure for skeletal elements, and to provide STR profiles to the Office of the Chief Medical Examiner (OCME) in New York City to be used for identification of the victims. A high throughput process was developed to include electronic accessioning of samples, so that the numbering system of the OCME was maintained; rapid preparation and sampling of skeletal fragments to allow for the processing of more than 250 fragments per day; use of a 96-well format for sample extraction, DNA quantification, and STR analysis; and use of the Applied Biosystems 3100 and 3700 instrumentation to develop STR profiles. Given the highly degraded nature of the skeletal remains received by Bode, an advanced DNA extraction procedure was developed to increase the quantity of DNA recovery and reduce the co-purification of polymerase chain reaction (PCR) amplification inhibitors. In addition, two new STR multiplexes were developed specifically for this project, which reduced the amplicon size of the STR loci, and therefore, enhanced the ability to obtain results from the most challenged of samples. In all, the procedures developed allowed for the analysis of more than 1,000 skeletal samples each week. Approximately 13,000 skeletal fragments were analyzed at least once, for a total of more than 18,000 analyses, and greater than 8,000 of the skeletal samples produced STR results (65%). The percentage of successful results was low in relation to previous mass fatality incidents involving airline disasters. However, when this same process was applied to the analysis of skeletal remains from the American Airlines Flight 587 disaster that occurred on November 12, 2001, the success rate was in line with expected results (ie, greater than 92% of the skeletal remains produced results). This illustrated the quality aspects of the procedure and the degree of degradation that had occurred for the remains of the WTC victims. For future mass fatality incidents, the quality, high throughput procedures developed by Bode will allow for more rapid DNA analysis of victim remains, more rapid identification of victims, and thus more rapid return of remains to family members.
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              Automated biometrics-based personal identification of the Hunter-Schreger bands of dental enamel.

              The use of automated biometrics-based personal identification systems is a ubiquitous procedure in present times. Biometrics has certain limitations, such as in cases when bodies are decomposed, burned, or only small fragments of calcified tissues remain. Dental enamel is the most mineralized tissue of organisms and resists post-mortem degradation. It is characterized by layers of prisms of regularly alternating directions, known as Hunter-Schreger bands (HSB). In this article, we show that the pattern variation of the HSB, referred here as toothprint, can be used as a biometric-based parameter for personal identification in automated systems.
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                Author and article information

                Journal
                J Forensic Dent Sci
                J Forensic Dent Sci
                JFDS
                Journal of Forensic Dental Sciences
                Medknow Publications & Media Pvt Ltd (India )
                0975-1475
                0975-2137
                Jan-Apr 2016
                : 8
                : 1
                : 28-31
                Affiliations
                [1] Department of Oral Medicine and Radiology, School of Dental Sciences, Sharda University, Greater Noida, India
                [1 ] Department of Oral Pathology, I.T.S Dental College, Ghaziabad, Uttar Pradesh, India
                [2 ] Department of Oral Medicine and Radiology, MS Ramaiah Dental College and Hospital, Bangalore, Karnataka, India
                Author notes
                Address for correspondence: Dr. Manjushree Juneja, Department of Oral Medicine and Radiology, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India. E-mail: manjushreeagarwal@ 123456gmail.com
                Article
                JFDS-8-28
                10.4103/0975-1475.176951
                4799516
                27051220
                e53fcc83-6cab-4289-9e88-02fb291c3c4f
                Copyright: © Journal of Forensic Dental Sciences

                This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

                History
                Categories
                Original Article

                Forensic science
                ameloglyphics,enamel rod end patterns,forensic odontology,tooth print
                Forensic science
                ameloglyphics, enamel rod end patterns, forensic odontology, tooth print

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