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      Objective comparison of particle tracking methods

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          Abstract

          The first community competition designed to objectively compare the performance of particle tracking algorithms provides valuable practical information for both users and developers. Supplementary information The online version of this article (doi:10.1038/nmeth.2808) contains supplementary material, which is available to authorized users.

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          The fluorescent toolbox for assessing protein location and function.

          Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted.
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            Algorithms for the Assignment and Transportation Problems

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              Single-particle tracking: applications to membrane dynamics.

              Measurements of trajectories of individual proteins or lipids in the plasma membrane of cells show a variety of types of motion. Brownian motion is observed, but many of the particles undergo non-Brownian motion, including directed motion, confined motion, and anomalous diffusion. The variety of motion leads to significant effects on the kinetics of reactions among membrane-bound species and requires a revision of existing views of membrane structure and dynamics.
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                Author and article information

                Journal
                Nature Methods
                Nat Methods
                Springer Science and Business Media LLC
                1548-7091
                1548-7105
                March 2014
                January 19 2014
                March 2014
                : 11
                : 3
                : 281-289
                Article
                10.1038/nmeth.2808
                29fdb35d-ff27-436c-b7cd-e7d93515a9d9
                © 2014

                https://creativecommons.org/licenses/by-nc-sa/3.0/

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