Systemic hyperaminoacidemia, induced by either intravenous amino acid infusion or protein ingestion, reduces insulin-stimulated glucose disposal. Studies in mice suggest that the valine metabolite 3-hydroxyisobutyrate (3-HIB), fibroblast growth factor 21 (FGF21), adiponectin, and non-esterified fatty acids (NEFA) may be involved in the amino acid-mediated insulin resistance. We therefore measured the rate of glucose disposal, and plasma 3-HIB, FGF21, adiponectin, and NEFA concentrations in 30 women during basal conditions and during a hyperinsulinemic-euglycemic clamp procedure (HECP) with and without concomitant ingestion of protein (n=15) or an amount of leucine that matched the amount in the protein (n=15). We found that during the HECP without protein or leucine ingestion, plasma 3-HIB concentration decreased (from 35 ± 2 to 14 ± 1 µmol/l; grand mean ± SEM) and FGF21 concentration increased (from 178 [116, 217] to 509 [340, 648] pg/ml; grand median [quartiles]). Protein, but not leucine, ingestion decreased insulin-stimulated glucose disposal (P<0.05) and prevented both the HECP-mediated decrease in 3-HIB and increase in FGF21 concentration in plasma. Neither protein nor leucine ingestion altered plasma adiponectin or NEFA concentrations. These findings suggest that 3-HIB and FGF21 might be involved in the protein-mediated insulin resistance in people.