1
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The enzymology and intracellular organization of peptide precursor processing: the secretory vesicle hypothesis.

      Neuroendocrinology

      Adrenal Medulla, metabolism, Amino Acid Sequence, Animals, Arginine Vasopressin, Cattle, Chromaffin Granules, Endopeptidases, Endoplasmic Reticulum, Exopeptidases, Golgi Apparatus, Hormones, Humans, Hydrogen-Ion Concentration, Mice, Organoids, enzymology, Oxytocin, Peptide Hydrolases, Pituitary Gland, Posterior, Pro-Opiomelanocortin, Protein Precursors, Rats

      Read this article at

      ScienceOpenPubMed
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The 'secretory vesicle hypothesis of precursor processing' states that the initial endopeptidase cleavages which excise the nascent, biologically active peptides from their protein precursors occur primarily in secretory vesicles (or granules). Hence, all the processing steps subsequent to these cleavages must also occur within these organelles. Two types of evidence are presented in support of this view: (1) cell biological studies which implicate the secretory vesicle as the site of precursor conversion to peptides, and (2) enzymological studies which locate and characterize putative processing enzymes in secretory vesicles. The processing enzymes reviewed include the 'prohormone-converting enzymes' which cleave at pairs of basic amino acids, other endopeptidases, carboxypeptidase-B-like enzymes and aminopeptidase, and N-acetylation and alpha-amidation enzymes. The properties of these enzymes in relation to the nature of the processing micro-environment in the secretory vesicles is discussed.

          Related collections

          Author and article information

          Journal
          3883214

          Comments

          Comment on this article