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      Identification of mRNAs Regulated by Interferon-γ in Cultured Rat Astrocytes by PCR Differential Display

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      Neuroimmunomodulation
      S. Karger AG

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          Abstract

          We have used RNA differential display to identify specific mRNA species that are regulated by interferon-γ (IFNγ) treatment of enriched astrocyte cultures. Following a 24-hour treatment with IFN, mRNA was subjected to differential display using 15 different random primers. 105 upregulated and 5 downregulated cDNAs were selected for further sequencing and identification. Northern blot analysis confirmed the upregulation of 13 genes identical or highly similar to: intercrine, adrenomedullin, H-revl07, CAP-like protein, ATP synthase Ε-subunit, complement C3, S-100β, Ca<sup>2+</sup> ATPase, mgl1, IFN-upregulated 56-kD protein mRNA, laminin receptor-like protein, protein tyrosine phosphatase, and zic. These data suggest that exposure to IFNγ results in a complex change in the pattern of astrocyte gene expression.

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          Author and article information

          Journal
          Neuroimmunomodulation
          Neuroimmunomodulation
          S. Karger AG
          1021-7401
          1423-0216
          July 1 2004
          1995
          July 12 1996
          : 2
          : 6
          : 347-355
          Article
          10.1159/000097214
          6823c7cb-f2b5-4c77-9171-e7eba6aff190
          © 1996

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