There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
We have used RNA differential display to identify specific mRNA species that are regulated
by interferon-γ (IFNγ) treatment of enriched astrocyte cultures. Following a 24-hour
treatment with IFN, mRNA was subjected to differential display using 15 different
random primers. 105 upregulated and 5 downregulated cDNAs were selected for further
sequencing and identification. Northern blot analysis confirmed the upregulation of
13 genes identical or highly similar to: intercrine, adrenomedullin, H-revl07, CAP-like
protein, ATP synthase Ε-subunit, complement C3, S-100β, Ca<sup>2+</sup> ATPase, mgl1,
IFN-upregulated 56-kD protein mRNA, laminin receptor-like protein, protein tyrosine
phosphatase, and zic. These data suggest that exposure to IFNγ results in a complex
change in the pattern of astrocyte gene expression.