Recent trends in click chemistry as a promising technology for virus-related research

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Abstract

Highlights • Click chemistry describes reactions used to generate substances by joining small units together with heteroatom linkages. • Biosensors combined with analytical devices is convenient strategy for viral detection. • We reviewed the recent applications of click reactions in virus-related research. • This review provides an overview of the general principles and applications of click chemistry in virus-related research.

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Thiol-ene click chemistry.

Charles Hoyle, Christopher Bowman (2010)

Following Sharpless' visionary characterization of several idealized reactions as click reactions, the materials science and synthetic chemistry communities have pursued numerous routes toward the identification and implementation of these click reactions. Herein, we review the radical-mediated thiol-ene reaction as one such click reaction. This reaction has all the desirable features of a click reaction, being highly efficient, simple to execute with no side products and proceeding rapidly to high yield. Further, the thiol-ene reaction is most frequently photoinitiated, particularly for photopolymerizations resulting in highly uniform polymer networks, promoting unique capabilities related to spatial and temporal control of the click reaction. The reaction mechanism and its implementation in various synthetic methodologies, biofunctionalization, surface and polymer modification, and polymerization are all reviewed.

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A chemical method for fast and sensitive detection of DNA synthesis in vivo.

Adrian Salic, Timothy J. Mitchison (2008)

We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the EdU label is highly sensitive and can be accomplished in minutes. The small size of the fluorescent azides used for detection results in a high degree of specimen penetration, allowing the staining of whole-mount preparations of large tissue and organ explants. In contrast to BrdU, the method does not require sample fixation or DNA denaturation and permits good structural preservation. We demonstrate the use of the method in cultured cells and in the intestine and brain of whole animals.