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Abstract
Hemodialysis (HD) patients are exposed to high oxidative stress, however, the nature
of this stress is still unclear. In this study, we employed a specific lipid peroxidative
product, phosphatidylcholine hydroperoxide (PCOOH), and evaluated the peroxidative
effect of end stage renal disease by measuring thiobarbituric acid reactive substances
(TBARS) and PCOOH in both plasma and erythrocyte membrane. We also surveyed plasma
TBARS and PCOOH before and after HD sessions thereby assessing oxidative stress by
a single HD procedure. The plasma TBARS level of healthy controls was 2.9 ± 0.4 nmol/ml.
Those of HD patients before and after HD session were 5.1 ± 1.4 and 3.1 ± 0.5 nmol/ml,
respectively, and the pre-HD plasma TBARS levels were significantly higher than those
of controls and after HD. The Plasma PCOOH concentration of patients before HD was
119.7 ± 58.4 pmol/ml and was significantly higher than that of controls which was
88.6 ± 14.3 pmol/ml. After HD, the plasma PCOOH level decreased to 103.2 ± 36.0 pmol/ml,
which was still significantly higher than that of controls. In erythrocytes, the PCOOH
level of patients was 259.3 ± 105.4 nmol/g RBC and was significantly higher than that
of controls with 88.6 ± 32.0 nmol/g RBC. Analyzed with respect to the cause of renal
disease, the polycystic kidney disease patients showed significantly lower plasma
PCOOH levels than the others. These results suggest that there is an increase of lipid
peroxidation in both plasma and erythrocytes of HD patients, though this oxidative
stress was not brought about by HD.