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      Detection of prepro-ET-1 mRNA in normal rat kidney by in situ RT-PCR.

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          Abstract

          Available evidence has shown that endothelin-1 (ET-1) acts in an autocrine/paracrine fashion rather than as a hormone or cytokine. Therefore, the analysis of local ET-1 production is a crucial step toward understanding its physiological and pathophysiological importance. In this study, in situ RT-PCR was utilized to detect tubular expression of prepro-ET-1 mRNA in normal rat kidney.

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          Most cited references6

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          Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons.

          Defects in the gene encoding the endothelin-B receptor produce aganglionic megacolon and pigmentary disorders in mice and humans. We report that a targeted disruption of the mouse endothelin-3 ligand (EDN3) gene produces a similar recessive phenotype of megacolon and coat color spotting. A natural recessive mutation that results in the same developmental defects in mice, lethal spotting (ls), failed to complement the targeted EDN3 allele. The ls mice carry a point mutation of the EDN3 gene, which replaces the Arg residue at the C-terminus of the inactive intermediate big EDN3 with a Trp residue. This mutation prevents the proteolytic activation of big EDN3 by ECE-1. These findings indicate that interaction of EDN3 with the endothelin-B receptor is essential in the development of neural crest-derived cell lineages. We postulate that defects in the human EDN3 gene may cause Hirschsprung's disease.
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            Molecular characterization of endothelin receptors

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              Presence of immunoreactive endothelin in human plasma.

              A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man.
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                Author and article information

                Journal
                Nephron Exp. Nephrol.
                Nephron. Experimental nephrology
                S. Karger AG
                1660-2129
                1660-2129
                2003
                : 95
                : 2
                Affiliations
                [1 ] Department of Hygiene and Preventive Medicine, Faculty of Medicine, Saitama Medical School, Tokyo University of Science, Saitama, Japan. kmorid@po.wind.ne.jp
                Article
                73672
                10.1159/000073672
                14610324
                2a6891d9-cc73-469d-96bf-8a771ff4f677
                History

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