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      Expression of AQP3 and AQP4 in colonic tissues of rats with hyperlipidemia and intervention effect of modified Rhizoma Alismatis decoction


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          Objective To examine effects of Rhizoma Alismatis decoction (RAD) on hyperlipidemia and expressions of aquaporin 3 (AQP3) and aquaporin 4 (AQP4) in colon tissues in rats.

          Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups: a blank and a model group with distilled water, a positive control group with simvastatins, and high-, moderate-, and low-dose groups with modified RAD by gavage once a day continuously for five weeks. All the rats were fed with high-fat diet except for those of the blank control with normal diet. At the end of the treatments, serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were detected and the expressions of AQP3 and AQP4 in colon tissues were detected with real-time reverse transcription PCR (RT-PCR) and Western blot.

          Results Compared to those in the blank control rats, serum TG (2.183 ± 0.204 mmol/L), TC (2.013 ± 0.250 mmol/L), LDL-C (1.267 ± 0.087 mmol/L) were significantly elevated but serum HDL-C (0.731 ± 0.085 mmol/L) was significantly reduced in in the model rats ( P < 0.05 for all). Compared to those in the blank control rats, serum TG (1.205 ± 0.139 mmol/L), TC (1.164 ± 0.197 mmol/L), LDL-C (0.787 ± 0.057 mmol/L) were decreased but serum HDL-C (0.904 ± 0.115 mmol/L) was increased significantly in the rats with high-dose modified RAD ( P < 0.05 for all). The expressions of AQP3 (0.944 ± 0.052) and AQP4 (0.701 ± 0.067) in colon tissues of the model rats increased significantly compared to those of the blank control rats (both P < 0.01); while, the expressions of AQP3 (0.502 ± 0.085) and AQP4 (0.366 ± 0.061) in colon tissues of the rats treated with high-dose modified RAD decreased significantly compared to those of the model rats (both P < 0.05).

          Conclusion Modified Rhizoma Alismatis decoction can regulate blood lipids in rats and the effect may be related to the regulated expressions of AQP3 and AQP4 in colon tissue


          【摘 要】 目的 观察泽泻汤加味方对高脂血症大鼠血脂及结肠组织水通道蛋白 3 和 4(AQP3、AQP4)基因、蛋白表达的影响。 方法 将 60 只雄性 SD 大鼠随机分为对照组、模型组、阳性对照组(辛伐他汀)及泽泻汤加味方高、中、低剂量组,采用喂饲高脂饲料方法复制大鼠模型,造模同时给予相应药物干预,连续 5 周;观察大鼠血清中甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量变化,运用 RT-PCR、 Western blot 方法检测大鼠结肠组织中 AQP3 和 AQP4 基因、蛋白表达情况。 结果 与对照组比较,模型组大鼠血清中 TG、TC、LDL-C 含量[分别为(2.183 ± 0.204)、(2.013 ± 0.250)、(1.267 ± 0.087)mmol/L]升高,HDL-C 含量[(0.731 ± 0.085)mmol/L]降低( P < 0.05);与模型组比较,高剂量泽泻汤加味方组大鼠血清中 TG、TC、LDL-C 含量[分别为(1.205 ± 0.139)、(1.164 ± 0.197)、(0.787 ± 0.057)mmol/L]下降,HDL-C 含量[(0.904 ± 0.115)mmol/L]升高( P < 0.05)。与对照组比较,模型组大鼠结肠组织中 AQP3 和 AQP4 蛋白表达水平[分别为(0.944 ± 0.052)、(0.701 ± 0.067)]升高( P < 0.01);与模型组比较,高剂量泽泻汤加味方组大鼠结肠组织中 AQP3 和 AQP4 蛋白表达水平[(0.502 ± 0.085)、(0.366 ± 0.061)]均降低,呈剂量效应关系( P < 0.05)。 结论 泽泻汤加味方具有调节血脂作用,其机制可能与结肠组织 AQP3 和 AQP4 蛋白表达调控有关。

          Author and article information

          Chinese Journal of Public Health
          Chinese Journal of Public Health (China )
          01 April 2019
          28 February 2018
          : 35
          : 4
          : 406-409
          [1] 1Hebei University of Chinese Medicine, Shijiazhuang, Hebei Province 050200, China
          Author notes
          *Corresponding author: Zhang Yi-xin, E-mail: hbzyx123@ 123456163.com
          © 2019 China Public Health Editorial Department

          This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License (CC BY-NC 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc/4.0/.

          Journal Article

          Medicine,Nutrition & Dietetics,Occupational & Environmental medicine,Health & Social care,Infectious disease & Microbiology,Public health
          aquaporin 4,modified Rhizoma alismatis decoction,hyperlipidemia,aquaporin 3


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