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      Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

      Analytical Biochemistry

      Solutions, Rats, isolation & purification, RNA, Phenols, Phenol, analysis, Mammary Glands, Animal, Hydrogen-Ion Concentration, Humans, Guanidines, Guanidine, Chloroform, Cell Line, Animals

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          Abstract

          A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

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          Journal
          10.1006/abio.1987.9999
          2440339

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