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      Rapid measurement of anticoagulant rodenticides in human blood and urine using online turbulent flow chromatography coupled with liquid chromatography–tandem mass spectrometry

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          Abstract

          Anticoagulant rodenticides (ARs) are widely used to control rodents. A method based on online turbulent flow chromatography (TFC) combined with LC-MS/MS has been established for rapid quantitative determination of eight ARs in human blood and urine. This method, which does not require time-consuming pre-processing steps, renders it especially suited for use in emergency poisoning cases. Sample preparation, including extraction, centrifugation, and filtration, was followed by online clean-up using TFC. The total run-time was within 13.5 min, including online purification, chromatographic separation, and re-equilibration of the TFC system. The parameters for sample extraction, purification, separation, and detection in this study were optimized separately. The linear regression coefficients of matrix-matched calibration standard curves established for quantification were greater than 0.9976. The limit of quantification (LOQ) for the method were found to be 0.3–3.0 ng/mL in human blood and 0.06–0.6 ng/mL in urine. The recoveries of spiked target compounds at different concentrations in human blood and urine were 91.8–111.9% and 86.9–105.3%, respectively. Inter- and intra-day precision values were both less than 12.5%, and the matrix effects of human blood and urine samples for ARs were 75.3–108.6% and 102.7–130.0%, respectively. This method had successfully applied to the emergency detection of ARs in biological samples of poisoned patients.

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          Author and article information

          Contributors
          Journal
          1326
          Acta Chromatographica
          AChrom
          Akadémiai Kiadó (Budapest )
          2083-5736
          29 October 2020
          Affiliations
          [1 ] Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Municipal Center for Disease Control and Prevention , Zhoushan, PR China
          [2 ] Daishan Center for Disease Control and Prevention , Daishan, PR China
          [3 ] School of Food and Pharmacy, Zhejiang Ocean University , Zhoushan, PR China
          Author notes
          [* ]Corresponding author. E-mail: fangli123@ 123456126.com
          Author information
          https://orcid.org/0000-0002-6684-5925
          Article
          10.1556/1326.2020.00846
          42d377bb-5f9e-479f-af99-ea4baf946aae
          © 2020 The Authors

          Open Access. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited, a link to the CC License is provided, and changes – if any – are indicated.

          History
          : 27 August 2020
          : 16 September 2020
          Page count
          Figures: 6, Tables: 4, Equations: 0, References: 31, Pages: 10
          Funding
          Funded by: Science and Technology Program of Zhoushan
          Award ID: 2021B
          Funded by: Medical Health Science and Technology Program of Zhoushan
          Award ID: 2020YA01
          Categories
          Original Research Paper

          Materials properties,Nanomaterials,Chemistry,Nanotechnology,Analytical chemistry,Thin films & surfaces
          urine,anticoagulant rodenticides,turbulent flow chromatography,human blood,poisoning

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