Neotropiella is a genus of springtails which can be of medium size (2 mm) or relatively long (5 mm). These springtails live in leaf litter, under the bark of dead trees, or in decomposing wood, mainly in the Neotropical Region, and are often collected by litter samples on Berlese funnels or by pitfall traps. Most species have been described based on relatively few specimens, and there is insufficient information of complete chaetotaxy study for several species.A new species within Neotropiella was discovered from recent pitfall trap collections in Peru. Neotropiella peruana sp. n., was taxonomically treated and studied under both phase contrast and scanning electron microscopy. It is similar to N. insularis from Brazil, but smaller with only 4 mandibular teeth (versus 5) and with well-developed unguis lateral teeth. Intraspecific variation of the new species is provided. We also present the first DNA barcodes for the genus.