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      Analysis of genetic mosaics in developing and adult Drosophila tissues.

      1 ,
      Development (Cambridge, England)
      The Company of Biologists

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          Abstract

          We have constructed a series of strains to facilitate the generation and analysis of clones of genetically distinct cells in developing and adult tissues of Drosophila. Each of these strains carries an FRT element, the target for the yeast FLP recombinase, near the base of a major chromosome arm, as well as a gratuitous cell-autonomous marker. Novel markers that carry epitope tags and that are localized to either the cell nucleus or cell membrane have been generated. As a demonstration of how these strains can be used to study a particular gene, we have analyzed the developmental role of the Drosophila EGF receptor homolog. Moreover, we have shown that these strains can be utilized to identify new mutations in mosaic animals in an efficient and unbiased way, thereby providing an unprecedented opportunity to perform systematic genetic screens for mutations affecting many biological processes.

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          Author and article information

          Journal
          Development
          Development (Cambridge, England)
          The Company of Biologists
          0950-1991
          0950-1991
          Apr 1993
          : 117
          : 4
          Affiliations
          [1 ] Howard Hughes Medical Institute, University of California, Berkeley 94720.
          Article
          10.1242/dev.117.4.1223
          8404527
          5cac6969-9b43-4c41-ab7f-fa1f251abf07
          History

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