25
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis.

      Nature
      Animals, B-Lymphocytes, metabolism, pathology, Cell Proliferation, Cell Transformation, Neoplastic, genetics, Chromatin, Chromatin Immunoprecipitation, Disease Progression, Down-Regulation, Female, Fibroblasts, cytology, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genome, Lymphoma, B-Cell, Male, Mice, Mitogens, pharmacology, Promoter Regions, Genetic, Proto-Oncogene Proteins c-myc, RNA, Messenger, biosynthesis, Transcription Factors, Transcription, Genetic, Up-Regulation

      Read this article at

      ScienceOpenPublisherPMC
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The c-myc proto-oncogene product, Myc, is a transcription factor that binds thousands of genomic loci. Recent work suggested that rather than up- and downregulating selected groups of genes, Myc targets all active promoters and enhancers in the genome (a phenomenon termed 'invasion') and acts as a general amplifier of transcription. However, the available data did not readily discriminate between direct and indirect effects of Myc on RNA biogenesis. We addressed this issue with genome-wide chromatin immunoprecipitation and RNA expression profiles during B-cell lymphomagenesis in mice, in cultured B cells and fibroblasts. Consistent with long-standing observations, we detected general increases in total RNA or messenger RNA copies per cell (hereby termed 'amplification') when comparing actively proliferating cells with control quiescent cells: this was true whether cells were stimulated by mitogens (requiring endogenous Myc for a proliferative response) or by deregulated, oncogenic Myc activity. RNA amplification and promoter/enhancer invasion by Myc were separable phenomena that could occur without one another. Moreover, whether or not associated with RNA amplification, Myc drove the differential expression of distinct subsets of target genes. Hence, although having the potential to interact with all active or poised regulatory elements in the genome, Myc does not directly act as a global transcriptional amplifier. Instead, our results indicate that Myc activates and represses transcription of discrete gene sets, leading to changes in cellular state that can in turn feed back on global RNA production and turnover.

          Related collections

          Author and article information

          Comments

          Comment on this article