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      Extracellular vesicle isolation and characterization: toward clinical application.

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          Abstract

          Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.

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          Author and article information

          Journal
          J. Clin. Invest.
          The Journal of clinical investigation
          1558-8238
          0021-9738
          Apr 1 2016
          : 126
          : 4
          Article
          81129
          10.1172/JCI81129
          4811150
          27035807
          9e739d06-5dfc-4ac5-8b91-5c17e6dea4a0
          History

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