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      Kinetics of IL-17- and interferon-gamma-producing PLPp-specific CD4 T cells in EAE induced by coinjection of PLPp/IFA with pertussis toxin in SJL mice.

      1 ,
      Neuroscience letters
      Elsevier BV

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          Abstract

          Systemic administration of Pertussis toxin (PTX) abrogates T cell tolerance mediated by injection of neuroantigens in incomplete Freund's adjuvant (IFA) and causes experimental autoimmune encephalomyelitis (EAE). PTX concomitantly induces high frequencies of neuroantigen-specific IFN-gamma- and IL-17-producing T cells. Both IL-17 and IFN-gamma have been implicated as a key effector cytokines in the pathogenesis of EAE, possibly with different functions. We therefore investigated potential differences in the temporal and spatial kinetics of the PTX-induced neuroantigen-specific IFN-gamma- and IL-17-producing T cell effector populations. IFN-gamma- and IL-17-producing PLPp-specific T cells initially arose in comparable frequencies in the local draining lymph nodes (drLN) after immunization as measured by cytokine ELISPOT. High frequencies of both IFN-gamma- and IL-17-producing T cells were present in the immune periphery before onset of EAE. The highest frequencies of PTX-induced IFN-gamma- and IL-17-producing PLPp-specific cells coincided in the inflamed CNS during acute EAE. During recovery, both IFN-gamma- and IL-17-producing PLPp-specific T cells simultaneously disappeared from the CNS, whereas high frequencies of these cells remained present in the immune periphery. The functional affinity of both IFN-gamma- and IL-17-producing T cells did not change during EAE. Therefore, autoimmune pathology in this model did not correlate with specific PTX effects either on Th1 or Th17 cells regarding their kinetics and CNS migration.

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          Author and article information

          Journal
          Neurosci. Lett.
          Neuroscience letters
          Elsevier BV
          1872-7972
          0304-3940
          Jun 07 2010
          : 476
          : 3
          Affiliations
          [1 ] Clinical Research Group for Multiple Sclerosis and Neuroimmunology, Department of Neurology, University of Würzburg, Würzburg, Germany. harald.hofstetter@uni-duesseldorf.de
          Article
          S0304-3940(10)00455-6
          10.1016/j.neulet.2010.04.018
          20398738
          6e9ba764-b9bb-40d1-87f5-3dd75c5c2cdf

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