A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry
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Abstract
A microwave-assisted extraction (MAE) and ultra high performance liquid chromatography
coupled with diode array detection and time-of-flight mass spectrometry (UHPLC-DAD-TOF-MS)
method was developed for simultaneous determination of 14 phenolic compounds in the
root of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth. Operational conditions
of MAE were optimized by central composite design (CCD). The optimized result was
65% ethanol as extraction solvent, 17mL of extraction volume, 100 degrees C of extraction
temperature and 2min of hold time. A Zorbax SB C(18) (50mmx4.6mm I.D., 1.8microm)
and gradient elution were used during the analysis. The chromatographic peaks of 14
investigated compounds in samples were successfully identified by comparing their
retention time, UV spectra and TOF mass data with the reference substances. All calibration
curves showed good linearity (r>0.9997) within the test ranges. The intra-day and
inter-day variations were less than 1.77% and 2.88%, respectively. The developed method
was successfully applied to determine the investigated compounds in 10 samples of
Radix Puerariae Lobatae and Radix Puerariae Thomsonii, respectively. The result indicated
that MAE and UHPLC-DAD-TOF-MS system might provide a rapid method for the quality
control of Radix Puerariae.
Published by Elsevier B.V.