A list of species of Hydrophilidae from Quindío Department, Colombia is presented. This list is based on recently collected specimens, a survey of data from the literature, and specimens deposited in the Colección de Insectos de laUniversidad del Quindío (CIUQ). Twenty-three species, including 16 new records from Colombia, are listed. Information about biology of each species and a checklist of the overall Colombian species are given.