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      Effect of thimerosal on thyroid hormones metabolism in rats

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          Abstract

          Mercury seems to exert an inhibitory effect on deiodinases, but there are few studies using Thimerosal (TM) as the mercury source. We aimed to elucidate the effect of TM on thyroid hormones peripheral metabolism. Adult Wistar female rats received 0.25 µg or 250 µg TM/100 g BW, IM, twice a week, for a month. We evaluated serum total T 3 and T 4, D1 activity using 125I-rT 3 as tracer, and D2 activity using 125I-T 4. NADPH oxidase activity was measured by Amplex-red/HRP method and mRNA levels by real time PCR. Serum T 4 was increased and T 3 decreased by the greatest dose of TM. Even though D1 activity in pituitary and kidney was reduced by the highest dose of TM, hepatic D1 activity and D1 mRNA levels remained unchanged. D2 activity was also significantly decreased by the highest dose of TM in all CNS samples tested, except cerebellum, but D2 mRNA was unaltered. mRNA levels of the tested NADPH oxidases were not affected by TM and NADPH oxidase activity was either unaltered or decreased. Our results indicate that TM might directly interact with deiodinases, inhibiting their activity probably by binding to their selenium catalytic site, without changes in enzyme expression.

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              Deiodinases: implications of the local control of thyroid hormone action.

              The deiodinases activate or inactivate thyroid hormone, and their importance in thyroid hormone homeostasis has become increasingly clear with the availability of deiodinase-deficient animals. At the same time, heightened interest in the field has been generated following the discovery that the type 2 deiodinase can be an important component in both the Hedgehog signaling pathway and the G protein-coupled bile acid receptor 1-mediated (GPBAR1-mediated) signaling cascade. The discovery of these new roles for the deiodinases indicates that tissue-specific deiodination plays a much broader role than once thought, extending into the realms of developmental biology and metabolism.
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                Author and article information

                Journal
                Endocr Connect
                Endocr Connect
                EC
                Endocrine Connections
                Bioscientifica Ltd (Bristol )
                2049-3614
                November 2017
                05 October 2017
                : 6
                : 8
                : 741-747
                Affiliations
                [1 ]Laboratório de Fisiologia Endócrina Doris Rosenthal Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
                [2 ]NUMPEX Pólo de Xerém, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
                Author notes
                Correspondence should be addressed to V M Corrêa da Costa; Email: vmccosta@ 123456biof.ufrj.br
                Article
                EC170220
                10.1530/EC-17-0220
                5670274
                29101249
                d4f84419-1804-49c9-a360-37b6a5368008
                © 2017 The authors

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 29 September 2017
                : 5 October 2017
                Categories
                Research

                thimerosal,thyroid function,iodothyronine deiodinases

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