Abortive transformation of rat 3Y1 cells by simian virus 40: Viral function overcoming inhibition of cellular proliferation under various conditions of culture
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Abstract
Resting cultures of nonpermissive rat 3Y1 cells were infected with simian and T antigen
expression and entry into S phase were examined under various conditions of culture.
In the complete absence of serum from the medium or at an extremely high cell density,
the cells delayed T antigen expression and entry into S phase, leaving the interval
between the two events constant. Results using the viral mutants deleted in the coding
region for the small t antigen ruled out the role of this antigen in induction of
S phase. From these and other results presented, we conclude that the large T antigen
induces S phase with the same efficiency under different conditions of cultures. We
also present the evidence that the large T antigen function is required and is sufficient
for entry into S phase in the second as well as in the first generation.