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      Laboratory layered latte

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          Abstract

          Inducing thermal gradients in fluid systems with initial, well-defined density gradients results in the formation of distinct layered patterns, such as those observed in the ocean due to double-diffusive convection. In contrast, layered composite fluids are sometimes observed in confined systems of rather chaotic initial states, for example, lattes formed by pouring espresso into a glass of warm milk. Here, we report controlled experiments injecting a fluid into a miscible phase and show that, above a critical injection velocity, layering emerges over a time scale of minutes. We identify critical conditions to produce the layering, and relate the results quantitatively to double-diffusive convection. Based on this understanding, we show how to employ this single-step process to produce layered structures in soft materials, where the local elastic properties vary step-wise along the length of the material.

          Abstract

          The ability to form density layering in a fluid in a simple and repeatable way is of relevance to a number of industrial and environmental processes. Here Xue et al. show formation of layers by simple injection of a hot liquid into a warm one at a predetermined critical pouring velocity.

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          Pattern formation outside of equilibrium

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            A synthetic multicellular system for programmed pattern formation.

            Pattern formation is a hallmark of coordinated cell behaviour in both single and multicellular organisms. It typically involves cell-cell communication and intracellular signal processing. Here we show a synthetic multicellular system in which genetically engineered 'receiver' cells are programmed to form ring-like patterns of differentiation based on chemical gradients of an acyl-homoserine lactone (AHL) signal that is synthesized by 'sender' cells. In receiver cells, 'band-detect' gene networks respond to user-defined ranges of AHL concentrations. By fusing different fluorescent proteins as outputs of network variants, an initially undifferentiated 'lawn' of receivers is engineered to form a bullseye pattern around a sender colony. Other patterns, such as ellipses and clovers, are achieved by placing senders in different configurations. Experimental and theoretical analyses reveal which kinetic parameters most significantly affect ring development over time. Construction and study of such synthetic multicellular systems can improve our quantitative understanding of naturally occurring developmental processes and may foster applications in tissue engineering, biomaterial fabrication and biosensing.
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              Recent Developments in Rayleigh-Bénard Convection

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                Author and article information

                Contributors
                hastone@princeton.edu
                Journal
                Nat Commun
                Nat Commun
                Nature Communications
                Nature Publishing Group UK (London )
                2041-1723
                12 December 2017
                12 December 2017
                2017
                : 8
                : 1960
                Affiliations
                [1 ]ISNI 0000 0001 2097 5006, GRID grid.16750.35, Department of Mechanical and Aerospace Engineering, , Princeton University, ; Princeton, NJ 08544 USA
                [2 ]ISNI 0000000121581746, GRID grid.5037.1, Linné Flow Centre and Swedish e-Science Research Centre (SeRC), , KTH Mechanics, ; Stockholm, SE 10044 Sweden
                [3 ]ISNI 0000 0001 2292 0500, GRID grid.37172.30, Department of Mechanical Engineering, , Korea Advanced Institute of Science and Technology, ; Deajeon, 34141 South Korea
                Author information
                http://orcid.org/0000-0002-4729-0741
                http://orcid.org/0000-0001-6273-9124
                http://orcid.org/0000-0002-3443-0709
                http://orcid.org/0000-0002-2393-723X
                http://orcid.org/0000-0002-9670-0639
                Article
                1852
                10.1038/s41467-017-01852-2
                5727143
                a8230415-f875-4e0c-a8a2-e5529c4b0e58
                © The Author(s) 2017

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 6 July 2017
                : 20 October 2017
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