In this study, we introduce a novel genus Paraeutypella of the family Diatrypaceae, comprising three species viz. Paraeutypella guizhouensis sp. nov. and P. citricola (basionym: Eutypella citricola) and P. vitis (basionym: Sphaeria vitis). Diatrypella longiasca sp. nov. is also introduced, which forms a distinct clade in Diatrypella sensu stricto. The discovery of this new genus will contribute to expand the knowledge and taxonomic framework of Diatrypaceae (Xylariales).Generic delimitations in Diatrypaceae, Xylariales, are unsettled because the phylogeny has yet to be resolved using extensive taxon sampling and sequencing of ex-type cultures. During an investigation of xylarialean fungi, we collected eutypella-like taxon which is distinct from Eutypella s. str. in our phylogenetic analyses (ITS and β-tubulin) thus, introduced as Paraeutypella guizhouensis gen. et sp. nov.. Paraeutypella is characterized by having 4–25 perithecia in a stroma each with 3–6 sulcate, long ostiolar necks. Paraeutypella citricola comb. nov. (basionym: Eutypella citricola) is introduced on Acer sp. from China. Diatrypella longiasca sp. nov. is introduced as a new species in Diatrypella s. str. which has 2–5 ascomata per stromata and long ascospores, unusual when compared to other Diatrypella species and distinct phylogenetically.