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      The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation.

      Cell

      Amino Acid Sequence, Animals, Base Sequence, Cell Line, Mice, Molecular Sequence Data, Molecular Weight, Neoplasm Proteins, chemistry, metabolism, Nuclear Proteins, Oncogene Proteins, isolation & purification, Plasmids, Protein Binding, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-mdm2, Rats, Sequence Homology, Nucleic Acid, Transcriptional Activation, drug effects, Tumor Suppressor Protein p53, genetics

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          Abstract

          A cellular phosphoprotein with an apparent molecular mass of 90 kd (p90) that forms a complex with both mutant and wild-type p53 protein has been characterized, purified, and identified. The protein was identified as a product of the murine double minute 2 gene (mdm-2). The mdm-2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. To determine if mdm-2 could modulate p53 transactivation, a p53-responsive element from the muscle creatine kinase gene was employed. A wild-type p53-expressing plasmid enhanced the expression of the p53-responsive element when cotransfected into cells that contain no endogenous p53. When a cosmid expressing mdm-2 was transfected with this p53-expressing plasmid, the transactivation of the p53-responsive element was inhibited. Thus, a product of the mdm-2 oncogene forms a tight complex with the p53 protein, and the mdm-2 oncogene can inhibit p53-mediated transactivation.

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          1535557

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