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      Correction to: Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue

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          Abstract

          Correction to: Diabetol Metab Syndr (2020) 12:66 10.1186/s13098-020-00573-9 Following publication of the original article [1], the authors identified an error in the caption of Fig. 2 and the in-text citation of Fig. 2. The error was that the descriptions for panel b and c were swapped. Fig. 2 Expression of differentiation markers in hASC, IPC and GPC by indirect immunofluorescence. The images shown are representative of n = 3 experiments, visualized by confocal microscopy. Nuclear staining was performed with Hoechst 1: 500 (blue). Markers analyzed were Pdx1 (red), Ngn3 (green), Insulin (green), Glucagon (red), CD44 (green) and Vimentin (green). a Markers analyzed in hASC. b Markers analyzed in GPC. c Markers analyzed in IPC. Scale bar = 20 μm. Pdx1 pancreatic and duodenal homeobox 1, Ngn3 neurogenin 3, Ins insulin, Gcg glucagon, Vim vimentin Caption, page 5 It currently reads “b Markers analyzed in IPC. c Markers analyzed in GPC.” It should read “b Markers analyzed in GPC. c Markers analyzed in IPC.” In-text citation, results section, page 5 It currently reads: “Moreover, IPC expressed insulin, which was not evident in hASC (Fig. 2b)”. It should read “Moreover, IPC expressed insulin, which was not evident in hASC (Fig. 2c)”. The figure with updated caption is published in this correction article.

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          Microencapsulation of cellular aggregates composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue

          Background In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of patients is still a challenge. For these reasons, it is necessary to explore new cellular sources and immunological isolation methods oriented to develop T1DM cell-based therapies. Aims We postulate human adipose-derived stem cell (hASC) as an adequate source to generate pancreatic islet cells in vitro, and to produce islet-like structures. Furthermore, we propose microencapsulation of these aggregates as an immunological isolation strategy. Methods hASC obtained from lipoaspirated fat tissue from human donors were differentiated in vitro to insulin (Ins) and glucagon (Gcg) producing cells. Then, insulin producing cells (IPC) and glucagon producing cells (GPC) were cocultured in low adhesion conditions to form cellular aggregates, and later encapsulated in a sodium alginate polymer. Expression of pancreatic lineage markers and secretion of insulin or glucagon in vitro were analyzed. Results The results show that multipotent hASC efficiently differentiate to IPC and GPC, and express pancreatic markers, including insulin or glucagon hormones which they secrete upon stimulation (fivefold for insulin in IPC, and fourfold for glucagon, compared to undifferentiated cells). In turn, calculation of the Feret diameter and area of cellular aggregates revealed mean diameters of ~ 80 µm, and 65% of the aggregates reached 4000 µm2 at 72 h of formation. IPC/GPC aggregates were then microencapsulated in sodium-alginate polymer microgels, which were found to be more stable when stabilized with Ba2+, yielding average diameters of ~ 300 µm. Interestingly, Ba2+-microencapsulated aggregates respond to high external glucose with insulin secretion. Conclusions The IPC/GPC differentiation process from hASC, followed by the generation of cellular aggregates that are later microencapsulated, could represent a possible treatment for T1DM.
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            Author and article information

            Contributors
            claudia.jaracancino@gmail.com
            foyarzuna@ciq.uchile.cl
            fcarrion@udd.cl
            esgonzalez.md@gmail.com
            claudio.cappellileon@gmail.com
            pcaviede@med.uchile.cl
            Journal
            Diabetol Metab Syndr
            Diabetol Metab Syndr
            Diabetology & Metabolic Syndrome
            BioMed Central (London )
            1758-5996
            19 August 2020
            19 August 2020
            2020
            : 12
            : 74
            Affiliations
            [1 ]GRID grid.443909.3, ISNI 0000 0004 0385 4466, Programa de Farmacología Molecular y Clínica, ICBM, Facultad de Medicina, , Universidad de Chile, ; Independencia 1027., Casilla 7, Clasificador Nº 7, 8389100 Santiago, Chile
            [2 ]GRID grid.443909.3, ISNI 0000 0004 0385 4466, Advanced Center of Chronic Diseases (ACCDiS), , Universidad de Chile, ; Santiago, Chile
            [3 ]GRID grid.443909.3, ISNI 0000 0004 0385 4466, Depto. de Ciencias y Tecnología Farmacéuticas, Facultad de Ciencias Químicas y Farmacéuticas, , Universidad de Chile, ; Santiago, Chile
            [4 ]GRID grid.412187.9, ISNI 0000 0000 9631 4901, Programa de Inmunología Traslacional, Facultad de Medicina, , Clínica Alemana Universidad del Desarrollo, ; Santiago, Chile
            [5 ]GRID grid.7119.e, ISNI 0000 0004 0487 459X, Laboratorio de Patología Molecular, Instituto de Bioquímica y Microbiología, Facultad de Ciencias, , Universidad Austral de Chile, ; Valdivia, Chile
            [6 ]GRID grid.443909.3, ISNI 0000 0004 0385 4466, Centro de Biotecnología y Bioingeniería (CeBiB), Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, , Universidad de Chile, ; Santiago, Chile
            Author information
            http://orcid.org/0000-0003-4462-7422
            Article
            581
            10.1186/s13098-020-00581-9
            7439678
            0030797b-d177-4685-a3e5-d332c754879f
            © The Author(s) 2020

            Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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            Nutrition & Dietetics
            Nutrition & Dietetics

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