This study aims to compare the accommodation microfluctuation before and after 5 mins of digital reading in the dark with night shift mode. Nineteen subjects were recruited using convenient sampling and a crossover design was used to compare the accommodation microfluctuations two viewing conditions. There was no significant difference in accommodation microfluctuations before and after 5-minute reading with night shift mode and without night shift mode. The insignificant findings in the accommodation microfluctuation comparison seemed to suggest that Night Shift Mode did not display as an advantage in controlling accommodation.