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      Use of Electrophoresis and Gel Filtration to Characterize Human Pituitary Somatotropin Preparations after Storage

      ,

      Hormone Research in Paediatrics

      S. Karger AG

      Human somatotropin, Electrophoresis, Gel filtration, Storage

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          Abstract

          Fresh and stored preparations of human pituitary somatotropin examined by different polyacrylamide gel electrophoresis techniques and by gel exclusion chromatography (Ultrogel) clearly demonstrated that the hormone undergoes a structural alteration during storage for 3 years. The formed aggregates were readily quantitated from the gel filtration profile and from polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE). According to gel filtration the amount of aggregates in fresh and stored hormone was determined to be 2 and 10%, respectively, while SDS-PAGE indicated 6 and 14%.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1979
          1979
          25 November 2008
          : 10
          : 2-3
          : 104-111
          Affiliations
          AB Kabi, Research Department, Stockholm
          Article
          178994 Horm Res 1979;10:104–111
          10.1159/000178994
          437688
          © 1979 S. Karger AG, Basel

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          Pages: 8
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