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      Chemical Constituents of Essential Oil from Lippia sidoides Cham. (Verbenaceae) Leaves Cultivated in Hidrolândia, Goiás, Brazil

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          Abstract

          Several studies involving the family Verbenaceae, occurring in the Brazilian Cerrado, have emphasized the popular use of many aromatic species. We highlight the use of Lippia sidoides Cham., known as “alecrim pimenta,” native to northeastern Brazil and northern Minas Gerais. Leaves of this species were collected in antropized Brazilian Cerrado area, in Hidrolândia, Goiás, and their essential oils were extracted by hydrodistillation in a Clevenger-type apparatus and thereafter analyzed GC/MS. Among the compounds identified in this study were the most abundant oxygenated monoterpenes, followed by sesquiterpenes hydrocarbons. The oxygenated monoterpene 1,8-cineole was the major constituent followed by isoborneol and bornyl acetate. The chemical composition of essential oil described in this paper differs from that described in the literature for L. sidoides found in its native environment, where the major constituents are thymol and carvacrol.

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          Identification of essential oil components by gás chromatography mass spectroscopy

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            Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens

            Dental caries and periodontal disease are associated with oral pathogens. Several plant derivatives have been evaluated with respect to their antimicrobial effects against such pathogenic microorganisms. Lippia sidoides Cham (Verbenaceae), popularly known as "Alecrim-pimenta" is a typical shrub commonly found in the Northeast of Brazil. Many plant species belonging to the genus Lippia yield very fragrant essential oils of potential economic value which are used by the industry for the commercial production of perfumes, creams, lotions, and deodorants. Since the leaves of L. sidoides are also extensively used in popular medicine for the treatment of skin wounds and cuts, the objective of the present study was to evaluate the composition and antimicrobial activity of L. sidoides essential oil. The essential oil was obtained by hydro-distillation and analyzed by GC-MS. Twelve compounds were characterized, having as major constituents thymol (56.7%) and carvacrol (16.7%). The antimicrobial activity of the oil and the major components was tested against cariogenic bacterial species of the genus Streptococcus as well as Candida albicans using the broth dilution and disk diffusion assays. The essential oil and its major components thymol and carvacrol exhibited potent antimicrobial activity against the organisms tested with minimum inhibitory concentrations ranging from 0.625 to 10.0 mg/mL. The most sensitive microorganisms were C. albicans and Streptococcus mutans. The essential oil of L. sidoides and its major components exert promising antimicrobial effects against oral pathogens and suggest its likely usefulness to combat oral microbial growth.
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              Database of the Amazon aromatic plants and their essential oils

              The aromatic flora of the Amazon has been inventoried for 30 years. In this sense, were made over 500 field trips to collect over 2500 plants and to obtain more than 2000 essential oils and aroma concentrates, all of them submitted to GC and GC-MS. This work led to the creation of a database for the aromatic plants of the Amazon, which catalogs general information about 1250 specimens. The database has allowed the publication of the chemical composition of the oils and aromas of more than 350 species, associated with a larger number of chemical types. The essential oils of many species offer optimum conditions for economic exploitation and use in national and international market of fragrances, cosmetics, agricultural and household pesticides.
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                Author and article information

                Journal
                Int J Anal Chem
                Int J Anal Chem
                IJAC
                International Journal of Analytical Chemistry
                Hindawi Publishing Corporation
                1687-8760
                1687-8779
                2012
                23 February 2012
                : 2012
                Affiliations
                1Programa de Pós-Graduação em Biologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001 970 Goiânia, GO, Brazil
                2Instituto de Ciências da Saúde, Universidade Paulista, Campus Flamboyant, 74845 090 Goiânia, GO, Brazil
                3Programa de Pós-Graduação em Ciências Farmacêuticas, Laboratório de Pesquisa de Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, 74605 220 Goiânia, GO, Brazil
                4Faculdade de Farmácia, Universidade Federal de Goiás, 74605 220 Goiânia, GO, Brazil
                5Instituto de Ciências Biológicas, Universidade Federal de Goiás, Campus Samambaia, 74001 970 Goiânia, GO, Brazil
                6Instituto de Química, Laboratório de Bioatividade Molecular, Universidade Federal de Goiás, Campus Samambaia, 74001 970 Goiânia, GO, Brazil
                Author notes
                *Sandra Ribeiro de Morais: sandrarmorais@ 123456hotmail.com

                Academic Editor: Norberto Peporine Lopes

                Article
                10.1155/2012/363919
                3296135
                22505916
                004a0de2-27f6-45f2-a2ea-ecf62f0f87a6
                Copyright © 2012 Sandra Ribeiro de Morais et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Categories
                Research Article

                Analytical chemistry
                Analytical chemistry

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