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      Lipopolysaccharide-lnduced Fos Expression in Hypothalamic Nuclei of Neonatal Rats

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          Abstract

          The inability of neonates to fully evoke the acute-phase reaction to infection is thought to be due in part to central nervous system immaturity. We used the expression of Fos protein to evaluate whether acute-phase reaction deficits in neonates may indeed be linked to unresponsiveness of brain regions that mediate the responses to infection in adult animals. In this study, we used lipopolysaccharide (LPS) as the infectious agent. Rats aged 0–1, 3, 6, 9, 12 and 15 days were divided into groups treated with low- or high-dose LPS (Escherichia coli; 50 and 500 µg/kg, respectively, i.p.) or pyrogen-free saline (PFS) i.p. Two hours after injection, the animals were deeply anesthetized, sacrificed, and their brains removed for Fos immunocytochemistry. Fos-like immunore-active (FLI) neurons in the preoptic area (POA), paraventricular nucleus of the hypothalamus (PVN), and organum vasculosum laminae terminalis (OVLT) were compared between the treatment and the age groups. The fore-brain was devoid of FLI neurons in 1-day-old rats, but FLI neurons were present at 3 days of age and continued to increase with age until 9 days after birth. There were no significant differences between the LPS- and PFS-treated groups until day 12 of age. At 12 and 15 days of age, FLI neurons in the PVN, medial preoptic and lateral preoptic nuclei, and the area surrounding the OVLT were greater in the LPS-treated animals. The expression appeared to be both age- and dose-dependent. These observations show that the rat brain structures that participate in the mediation of the acute-phase reaction do not become responsive to systemic pyrogens until 12 days of age, thus suggesting that insensitivity of the brain to pyrogenic agents may be partly responsible for the poor response of neonates to infectious agents.

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          Author and article information

          Journal
          NIM
          Neuroimmunomodulation
          10.1159/issn.1021-7401
          Neuroimmunomodulation
          S. Karger AG
          1021-7401
          1423-0216
          1995
          1995
          02 May 1996
          : 2
          : 5
          : 282-289
          Affiliations
          Departments of aPhysical Therapy and bPhysiology and Biophysics, University of Tennessee, Memphis, Tenn., USA
          Article
          97207 Neuroimmunomodulation 1995;2:282–289
          10.1159/000097207
          8739201
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Categories
          Original Paper

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