For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
29
views
20
references
 Top references
cited by
3
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      463
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Could A Deletion in Neuraminidase Stalk Strengthen Human Tropism of the Novel Avian Influenza Virus H7N9 in China, 2013?

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Objective. A novel avian influenza A virus (AIV) H7N9 subtype which emerged in China in 2013 caused worldwide concern. Deletion of amino-acids 69 to 73 in the neuraminidase stalk was its most notable characteristic. This study is aimed to discuss the tropism and virulence effects of this deletion. Methods: Neuraminidase gene sequences of N9 subtype were collected from NCBI and GISAID. MEGA6.0, Stata12.0, and UCSF Chimera were employed for sequence aligning, significance testing, and protein tertiary structure homology modeling. Results: A total of 736 sequences were obtained; there were 81 human isolates of the novel AIV H7N9, of which 79 had the deletion. Among all the 654 avian origin sequences, only 43 had the deletion ( p < 0.001). Tertiary structure displayed that the deletion obviously changed the spatial direction of neuraminidase. Conclusions: The deletion in neuraminidase stalk could have strengthened human tropism of the novel AIV H7N9, as well as its virulence.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: found
          • Article: not found

          A single amino acid in the PB2 gene of influenza A virus is a determinant of host range.

          E Subbarao, W. London, B R Murphy (1993)
          The single gene reassortant virus that derives its PB2 gene from the avian influenza A/Mallard/NY/78 virus and remaining genes from the human influenza A/Los Angeles/2/87 virus exhibits a host range restriction (hr) phenotype characterized by efficient replication in avian tissue and failure to produce plaques in mammalian Madin-Darby canine kidney cells. The hr phenotype is associated with restriction of viral replication in the respiratory tract of squirrel monkeys and humans. To identify the genetic basis of the hr phenotype, we isolated four phenotypic hr mutant viruses that acquired the ability to replicate efficiently in mammalian tissue. Segregational analysis indicated that the loss of the hr phenotype was due to a mutation in the PB2 gene itself. The nucleotide sequences of the PB2 gene of each of the four hr mutants revealed that a single amino acid substitution at position 627 (Glu-->Lys) was responsible for the restoration of the ability of the PB2 single gene reassortant to replicate in Madin-Darby canine kidney cells. Interestingly, the amino acid at position 627 in every avian influenza A virus PB2 protein analyzed to date is glutamic acid, and in every human influenza A virus PB2 protein, it is lysine. Thus, the amino acid at residue 627 of PB2 is an important determinant of host range of influenza A viruses.
          Article 0 comments Cited 312 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Epidemiology of Human Infections with Avian Influenza A(H7N9) Virus in China

            Qun Li, Lei Zhou, Minghao Zhou … (2014)
            New England Journal of Medicine, 370(6), 520-532
            Article 0 comments Cited 286 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus.

              James Stevens, Adam L. Corper, Christopher Basler … (2004)
              The 1918 "Spanish" influenza pandemic represents the largest recorded outbreak of any infectious disease. The crystal structure of the uncleaved precursor of the major surface antigen of the extinct 1918 virus was determined at 3.0 angstrom resolution after reassembly of the hemagglutinin gene from viral RNA fragments preserved in 1918 formalin-fixed lung tissues. A narrow avian-like receptor-binding site, two previously unobserved histidine patches, and a less exposed surface loop at the cleavage site that activates viral membrane fusion reveal structural features primarily found in avian viruses, which may have contributed to the extraordinarily high infectivity and mortality rates observed during 1918.
              Article 0 comments Cited 150 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Paul B. Tchounwou: Role: Academic Editor
                Journal
                Journal ID (nlm-ta): Int J Environ Res Public Health
                Journal ID (iso-abbrev): Int J Environ Res Public Health
                Journal ID (publisher-id): ijerph
                Title: International Journal of Environmental Research and Public Health
                Publisher: MDPI
                ISSN (Print): 1661-7827
                ISSN (Electronic): 1660-4601
                Publication date (Electronic): 20 January 2015
                Publication date (Print): January 2015
                Volume: 12
                Issue: 1
                Pages: 1020-1028
                Affiliations
                [1 ]Department of Sanitary Microbiology, School of Public Health, Fudan University, Shanghai 200032, China; E-Mails: chenliangfd@ 123456sina.com (L.C.); jianglufang@ 123456fudan.edu.cn (L.J.)
                [2 ]Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China; E-Mails: epistat@ 123456gmail.com (Z.Z.); gmzhao@ 123456shmu.edu.cn (G.Z.); xiongchenglong1@ 123456sina.com (Q.J.)
                [3 ]Intensive Care Unit of Burn and Trauma Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; E-Mail: z8160720@ 123456126.com
                [4 ]Department of Epidemiology and Biostatistics, School of Public Health, Fudan University, Shanghai 200032, China
                [5 ]Department of Epidemiology and Community Medicine, Faculty of Medicine, University of Ottawa, Ontario K1H 8M5, Canada; E-Mail: ychen@ 123456uottawa.ca
                Author notes
                [†]

                These authors contributed equally to this work.

                [* ]Author to whom correspondence should be addressed; E-Mail: xiongchenglong@ 123456fudan.edu.cn ; Tel.: +86-021-5423-7237; Fax: +86-021-5423-7435.
                Article
                Publisher ID: ijerph-12-01020
                DOI: 10.3390/ijerph120101020
                PMC ID: 4306908
                PubMed ID: 25608590
                SO-VID: 0076d693-1ef4-41d2-a10c-dfde222bbed8
                Copyright © © 2015 by the authors; licensee MDPI, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 25 September 2014
                Date accepted : 13 January 2015
                Categories
                Subject: Article

                ScienceOpen disciplines: Public health
                Keywords: deletion,neuraminidase,h7n9,china
                Data availability:
                ScienceOpen disciplines: Public health
                Keywords: deletion, neuraminidase, h7n9, china

                Comments

                Comment on this article

                scite_

                Similar content463

                See all similar

                Cited by3

                See all cited by

                Most referenced authors545

                See all reference authors