• Record: found
  • Abstract: found
  • Article: not found

Activation of melanogenesis by vacuolar type H(+)-ATPase inhibitors in amelanotic, tyrosinase positive human and mouse melanoma cells.

Febs Letters

Animals, Vacuolar Proton-Translocating ATPases, Tumor Cells, Cultured, Time Factors, Reverse Transcriptase Polymerase Chain Reaction, metabolism, genetics, RNA, Messenger, antagonists & inhibitors, Proton-Translocating ATPases, Protein Processing, Post-Translational, Monophenol Monooxygenase, Mice, drug effects, Melanosomes, pathology, enzymology, Melanoma, biosynthesis, Melanins, Macrolides, Hydrogen-Ion Concentration, Humans, pharmacology, Enzyme Inhibitors, Enzyme Activation, Dose-Response Relationship, Drug, Cycloheximide, Anti-Bacterial Agents

Read this article at

      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


      In this study, we describe the activation of melanogenesis by selective vacuolar type H(+)-ATPase inhibitors (bafilomycin A1 and concanamycin A) in amelanotic human and mouse melanoma cells which express tyrosinase but show no melanogenesis. Addition of the inhibitors activated tyrosinase within 4 h, and by 24 h the cells contained measurable amounts of melanin. These effects were not inhibited by cycloheximide (2 microgram/ml) which is consistent with a post-translational mechanism of activation. Our findings suggest that melanosomal pH could be an important and dynamic factor in the control of melanogenesis in mammalian cells.

      Related collections

      Author and article information



      Comment on this article