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      Efecto interactivo del alimento y la calidad de agua en el crecimiento y sobrevivencia de postlarvas de acocil de río Cambarellus montezumae Translated title: Interactive effect of food and water quality on growth and survival of the crayfish Cambarellus montezumae

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          Abstract

          El objetivo de esta investigación es determinar el efecto del tipo de alimento -uno vivo, representado por Daphnia pulex y uno balanceado de la marca Purina Camaronina 35 con 35% de proteínas- y la calidad del agua -presencia y ausencia de microorganismos, y sólidos suspendidos- en el crecimiento y sobrevivencia de postlarvas del crustáceo Cambarellus montezumae. Para lo cual, se formaron 4 tratamientos que se evaluaron durante 14 semanas, mediante la realización de observaciones y biometrías periódicas como seguimiento del desarrollo en los organismos. Los datos obtenidos registraron diferencias significativas entre tratamientos (p< 0.05), en donde los mejores resultados de crecimiento y sobrevivencia se obtuvieron en los organismos alimentados con el alimento balanceado (M2 y M4), independientemente de la calidad del agua. Además, se obtuvieron valores de proteínas de hasta 45% y en la fracción lipídica se identificaron los ácidos grasos 20:4 n6 (ARA), 20:5 n3 (EPA) y 22:6 n3 (DHA) al considerar el cuerpo total del organismo. Lo antes mencionado incrementa el interés por el cultivo sustentable de C. montezumae para ser utilizado en la industria alimentaria a través de su adición en productos alimentarios de consumo humano debido a su contenido de biomoléculas de importancia biológica.

          Translated abstract

          The objective of this research was to determine the effect of the type of food -a line one represented by Daphnia pulex and a one commercial Camaronina 35 by Purina with 35% protein- and water quality -presence and absence of microorganisms and suspended solids- on growth and survival of postlarvae of the crustacean Cambarellus montezumae. Four treatments were evaluated for 14 weeks conducting observations and periodic measurements as a follow-up of the development of the organisms. The obtained data showed significant differences between treatments (p< 0.05), where the best results in growth and survival were obtained in the organisms fed on animal feed (M2 and M4) regardless of the water quality. In addition, values of up to 45% protein were obtained and the lipid fraction identified as the fatty acids 20:4 n6 (ARA), 20:5 n3 (EPA) and 22:6 n3 (DHA) when considering the total body of the organisms. Our results increase the interest in the sustainable culture of C. montezumae to be used in the food industry through its addition to food products for human consumption due to its content of biomolecules of biological importance.

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          Protein measurement with the Folin phenol reagent

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            Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids.

            The enzymes that are involved in the elongation of fatty acids differ in terms of the substrates on which they act. To date, the enzymes specifically involved in the biosynthesis of polyunsaturated fatty acids have not yet been identified. In an attempt to identify a gene(s) encoding an enzyme(s) specific for the elongation of gamma-linolenic acid (GLA) (18:3n-6), a cDNA expression library was made from the fungus Mortierella alpina. The cDNA library constructed in a yeast expression vector was screened by measuring the expressed elongase activity [conversion of GLA to dihomo-GLA (20:3n-6)] from an individual yeast clone. In this report, we demonstrate the isolation of a cDNA (GLELO) whose encoded protein (GLELOp) was involved in the conversion of GLA to dihomo-GLA in an efficient manner (60% conversion). This cDNA contains a 957-nucleotide ORF that encodes a protein of 318 amino acids. Substrate specificity analysis revealed that this fungal enzyme acted also on stearidonic acid (18:4n-3). This report identifies and characterizes an elongase subunit that acts specifically on the two Delta6-desaturation products, 18:3n-6 and 18:4n-3. When this GLELO cDNA was coexpressed with M. alpina Delta5-desaturase cDNA in yeast, it resulted in the conversion of GLA to arachidonic acid (20:4n-6) as well as the conversion of stearidonic acid to eicosopentaenoic acid (20:5n-3). Thus, this GLELO gene may play an critical role in the bio-production of both n-6 and n-3 polyunsaturated fatty acids.
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              Effects of nitrogen source and growth phase on proximate biochemical composition, lipid classes and fatty acid profile of the marine microalga Isochrysis galbana

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rmbiodiv
                Revista mexicana de biodiversidad
                Rev. Mex. Biodiv.
                Instituto de Biología (México )
                2007-8706
                2015
                : 86
                : 1
                : 131-142
                Affiliations
                [1 ] Instituto Tecnológico Superior del Occidente del Estado de Hidalgo MX
                [2 ] Centro de Estudios Tecnológicos, Aguas Continentales MX
                Article
                S1870-34532015000100131
                10.7550/rmb.48502
                00be42fd-eb6e-48a9-ad0b-b6b1d613c973
                Product
                Product Information: website
                Categories
                Biodiversity Conservation

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