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      Competitive RT-PCR to quantify CFTR mRNA in human endometrium.

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          Abstract

          Because the down-regulation by progesterone of cystic fibrosis transmembrane conductance regulator (CFTR) expression could be a useful specific marker to define the state of implant receptivity in endometrium, a competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed for quantifying the CFTR mRNA concentration in human endometrial samples. A competitor RNA was constructed with the same sequence as the CFTR sequence except for a 20-nucleotide insertion in the middle. The amplified products were separated by polyacrylamide gel electrophoresis. The ratio of CFTR band areas to competitor band areas provided the basis of quantification. Using this competitive RT-PCR, we measured CFTR mRNA in human endometrial samples taken at different periods of the menstrual cycle, in endometriosis, and in hyperplasia. Results show that the method is suitable for measuring the concentration of CFTR mRNA.

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          Author and article information

          Journal
          Clin. Chem.
          Clinical chemistry
          0009-9147
          0009-9147
          Nov 1996
          : 42
          : 11
          Affiliations
          [1 ] Service de Biochimie, Centre Hospitalier Universitaire, Besançon, France.
          Article
          8906074
          00d05c0d-5b3d-4d33-8585-580250e040e3
          History

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