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      Genetic heterogeneity in the species Leishmania tropica revealed by different PCR-based methods

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          Balancing selection at allozyme loci in oysters: implications from nuclear RFLPs.

          Population genetic analyses that depend on the assumption of neutrality for allozyme markers are used widely. Restriction fragment length polymorphisms in nuclear DNA of the American oyster evidence a pronounced population subdivision concordant with mitochondrial DNA. This finding contrasts with a geographic uniformity in allozyme frequencies previously thought to reflect high gene flow mediated by the pelagic gametes and larvae. The discordance likely is due to selection on protein electrophoretic characters that balances allozyme frequencies in the face of severe constraints to gene flow. These results raise a cautionary note for studies that rely on assumptions of neutrality for allozyme markers.
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            Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing

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              Visceral infection caused by Leishmania tropica in veterans of Operation Desert Storm.

              Visceral leishmaniasis, usually caused by Leishmania donovani, has rarely been reported from eastern Saudi Arabia, so it was not expected to affect the soldiers of Operation Desert Storm. We evaluated eight soldiers with visceral leishmanial infection, examining their serum with an immunofluorescent-antibody assay, examining their marrow or biopsy tissue for amastigotes with an indirect immunofluorescent-monoclonal-antibody assay, and culturing the parasites. Cultured promastigotes were isolated and characterized by isoenzyme analysis. None of the eight soldiers had classic signs or symptoms of visceral leishmaniasis (kala-azar). Seven soldiers had unexplained fever, chronic fatigue, malaise, cough, intermittent diarrhea, or abdominal pain that began up to seven months after they returned to the United States; one had no symptoms. Five had adenopathy or mild, transient hepatosplenomegaly. None had cutaneous manifestations. Diagnoses were made by bone marrow aspiration (seven patients) or lymph-node biopsy (one patient). Six isolates have been identified as L. tropica, which usually causes only cutaneous disease. Of the six patients treated with sodium stibogluconate, five improved and one remained symptomatic. L. tropica can produce visceral infection that can cause unexplained systemic illness in persons returning from areas where this organism is endemic.
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                Author and article information

                Journal
                Transactions of the Royal Society of Tropical Medicine and Hygiene
                Transactions of the Royal Society of Tropical Medicine and Hygiene
                Elsevier BV
                00359203
                March 2001
                March 2001
                : 95
                : 2
                : 217-224
                Article
                10.1016/S0035-9203(01)90173-7
                11355565
                00ea853a-1f28-4c81-b782-4b014b10b7ba
                © 2001
                History

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