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Sequential blood samples collected from mice infected perorally with an avirulent
strain of T. gondii were analysed for parasite DNA by a polymerase chain reaction
method (PCR). Two pairs of primers specific for gene B1 and the repetitive DNA sequence
TGR1E were used for DNA amplification. Amplified products were detected by means of
electrophoresis with ethidium bromide staining. Parasitemia was also determined by
cell culture. Parasitemia was never detected by the tissue culture method, whereas
parasite DNA was continuously detected with PCR from day 2 to day 21. These results
confirm the high sensitivity of PCR for T. gondii DNA in blood, and show that circulating
DNA is present for long periods in mice following primary infection.