Scientific Reports
6: Article number: 2554510.1038/srep25545; published online: 05
06
2016; updated: 03
28
2017
This Article contains errors in the Figure legends of Figure 1 and Figure 2. The correct
Figure legends appear below
Figure 1. The location and density of GPV antigen, CD4 and CD8α molecules in the liver
(LI), lung (LU), small intestine (SI), and rectum (R). Geese were humanly killed 5
days post infection by viruses. The protein locations in the different tissues of
GPV-infected birds were detected by IHC assay. Positive virus signals were detected,
cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical
staining, and sections were counterstained with haematoxylin. Mouse polyclonal antibody
against GPV was prepared by our laboratory. The dilution folds of mouse anti-duck
monoclonal CD4 antibodies (AbD Serotec MCA2478) and mouse anti-goose monoclonal CD8α
(provided by our laboratory) antibodies were 1:100, respectively. Incubation with
goat anti-mouse or goat anti-rabbit secondary antibody was performed according to
the protocols of the immunoassay kit. Liver (A,E,I), lung (B,F,J), small intestine
(C,G,K) and rectum (D,H,L).
Figure 2. The location and density of TMUV antigen, CD4 and CD8α molecule in the liver
(LI), brain (B), spleen (SP), and small intestine (SI). Geese were humanly killed
5 days post infection by viruses. These protein locations in the different tissues
of TMUV-infected birds were detected by IHC assay. Positive virus signals were detected,
cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical
staining, and sections were counterstained with haematoxylin. Rabbit polyclonal antibody
against TUMV E protein was prepared by our laboratory. The dilution folds of mouse
anti-duck monoclonal CD4 antibodies and mouse anti-goose monoclonal CD8α antibodies
were both 1:100. Incubation of goat anti-mouse or goat anti-rabbit secondary antibody
was performed by the protocols of the immunoassay kit. Liver (A,E,I), brain (B,F,J),
spleen (C,G,K) and small intestine (D,H,L).