+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Fatty Acid Composition of Developing Sea Buckthorn ( Hippophae rhamnoides L.) Berry and the Transcriptome of the Mature Seed

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.



          Sea buckthorn ( Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18∶2ω-6) and α-linolenic (18∶3ω-3) acids, and pulp oil that contains high levels of monounsaturated palmitoleic acid (16∶1ω-7). Sea buckthorn is fast gaining popularity as a source of functional food and nutraceuticals, but currently has few genomic resources; therefore, we explored the fatty acid composition of Canadian-grown cultivars (ssp. mongolica) and the sea buckthorn seed transcriptome using the 454 GS FLX sequencing technology.


          GC-MS profiling of fatty acids in seeds and pulp of berries indicated that the seed oil contained linoleic and α-linolenic acids at 33–36% and 30–36%, respectively, while the pulp oil contained palmitoleic acid at 32–42%. 454 sequencing of sea buckthorn cDNA collections from mature seeds yielded 500,392 sequence reads, which identified 89,141 putative unigenes represented by 37,482 contigs and 51,659 singletons. Functional annotation by Gene Ontology and computational prediction of metabolic pathways indicated that primary metabolism (protein>nucleic acid>carbohydrate>lipid) and fatty acid and lipid biosynthesis pathways were highly represented categories. Sea buckthorn sequences related to fatty acid biosynthesis genes in Arabidopsis were identified, and a subset of these was examined for transcript expression at four developing stages of the berry.


          This study provides the first comprehensive genomic resources represented by expressed sequences for sea buckthorn, and demonstrates that the seed oil of Canadian-grown sea buckthorn cultivars contains high levels of linoleic acid and α-linolenic acid in a close to 1∶1 ratio, which is beneficial for human health. These data provide the foundation for further studies on sea buckthorn oil, the enzymes involved in its biosynthesis, and the genes involved in the general hardiness of sea buckthorn against environmental conditions.

          Related collections

          Most cited references 64

          • Record: found
          • Abstract: found
          • Article: not found

          TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets.

          TGICL is a pipeline for analysis of large Expressed Sequence Tags (EST) and mRNA databases in which the sequences are first clustered based on pairwise sequence similarity, and then assembled by individual clusters (optionally with quality values) to produce longer, more complete consensus sequences. The system can run on multi-CPU architectures including SMP and PVM.
            • Record: found
            • Abstract: found
            • Article: not found

            Source to sink: regulation of carotenoid biosynthesis in plants.

            Carotenoids are a diverse group of colourful pigments naturally found in plants, algae, fungi and bacteria. They play essential roles in development, photosynthesis, root-mycorrhizal interactions and the production of phytohormones, such as abscisic acid and strigolactone. Carotenoid biosynthesis is regulated throughout the life cycle of a plant with dynamic changes in composition matched to prevailing developmental requirements and in response to external environmental stimuli. There are key regulatory nodes in the pathway that control the flux of metabolites into the pathway and alter flux through the pathway. The molecular nature of the mechanisms regulating carotenoid biosynthesis, including evidence for metabolite feedback, transcription and epigenetic control as well as their accumulation, storage and degradation will be the focus of this review. 2010 Elsevier Ltd. All rights reserved.
              • Record: found
              • Abstract: not found
              • Article: not found

              Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics.


                Author and article information

                Role: Editor
                PLoS One
                PLoS ONE
                PLoS ONE
                Public Library of Science (San Francisco, USA )
                27 April 2012
                : 7
                : 4
                [1 ]Department of Biology, University of Western Ontario, London, Ontario, Canada
                [2 ]Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Alberta, Canada
                [3 ]Agroforestry Development Centre, Agriculture and Agri-Food Canada, Indian Head, Saskatchewan, Canada
                [4 ]Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada
                [5 ]Departments of Computing Science and Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
                Max Planck Institute for Chemical Ecology, Germany
                Author notes

                Conceived and designed the experiments: PK RJW TF CS. Performed the experiments: TF CS. Analyzed the data: TF CS PK RJW. Contributed reagents/materials/analysis tools: WRS. Wrote the paper: PK TF CS. Oversaw standardization of fatty acid analysis: DW. Oversaw fatty acid analysis: RJW. Carried out in silico analysis of ESTs related to fatty acid biosynthesis in seeds of sea buckthorn, flax and Arabidopsis: DC RD.

                Fatima et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
                Page count
                Pages: 18
                Research Article
                Fatty Acids
                Computational Biology
                Genome Analysis Tools
                Developmental Biology
                Plant Growth and Development
                Genome Analysis Tools
                Gene Ontologies
                Genome Expression Analysis
                Genome Sequencing
                Plant Science



                Comment on this article