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      Cortical Na +,K +-ATPase Activity, Abundance and Distribution after in vivo Renal Ischemia without Reperfusion in Rats

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          Abstract

          The aim of our work was to study the changes in activity, abundance and distribution of sodium, potassium-adenosine triphosphatase (Na<sup>+</sup>,K<sup>+</sup>-ATPase) in membranes of cortical tubular cells in an in vivo model of ischemic injury without reperfusion. Na<sup>+</sup>,K<sup>+</sup>-ATPase, alkaline phosphatase (AP) activities and their distribution in membranes isolated from renal cortex using a Percoll gradient were studied after different ischemic periods. Na<sup>+</sup>,K<sup>+</sup>-ATPase α-subunit protein abundance was analysed by Western-blot. Plasma urea and cortical adenosine 5’triphosphate (ATP) were also measured. In cortical homogenates 5 min of ischemia promoted a diminution in ATP content. Na<sup>+</sup>,K<sup>+</sup>-ATPase activity diminished after 40 min and AP after 100 min of ischemia. Na<sup>+</sup>,K<sup>+</sup>-ATPase activity in the Percoll gradient fractions after 5 min peaked at a higher density and was significantly decreased after 40 min. AP activity was decreased in typically enriched apical membranes after both times of ischemia. At each time studied Na<sup>+</sup>,K<sup>+</sup>-ATPase abundance was increased in cortical homogenates and membranes. Our results showed opposite effects of ischemia on Na<sup>+</sup>,K<sup>+</sup>-ATPase activity and abundance. Increased levels of Na<sup>+</sup>,K<sup>+</sup>-ATPase protein were observed. The enzyme would be rapidly delivered to membrane domains and become inactivated as ischemia persists.

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          Alterations in epithelial polarity and the pathogenesis of disease states.

          The establishment and maintenance of epithelial-cell polarity are prerequisites for normal epithelial-cell and organ function. Knowledge of the processes involved in cell polarity has provided insight into the mechanisms of cell dysfunction and the pathogenesis of several diseases. These insights should lead to the development of specific strategies aimed at preventing or minimizing the progression of these diseases.
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            Phosphorylation of the Catalyic α-Subunit Constitutes a Triggering Signal for Na+,K+-ATPase Endocytosis

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              Author and article information

              Journal
              NEF
              Nephron
              10.1159/issn.1660-8151
              Nephron
              S. Karger AG
              1660-8151
              2235-3186
              2001
              2001
              24 August 2001
              : 89
              : 1
              : 82-89
              Affiliations
              Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, República Argentina
              Article
              46048 Nephron 2001;89:82–89
              10.1159/000046048
              11528237
              © 2001 S. Karger AG, Basel

              Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

              Page count
              Figures: 2, Tables: 1, References: 44, Pages: 8
              Product
              Self URI (application/pdf): https://www.karger.com/Article/Pdf/46048
              Categories
              Original Paper

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