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      zucchini and squash Encode Two Putative Nucleases Required for rasiRNA Production in the Drosophila Germline

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      Developmental Cell
      Elsevier BV

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          Abstract

          RNAi is a widespread mechanism by which organisms regulate gene expression and defend their genomes against viruses and transposable elements. Here we report the identification of Drosophila zucchini (zuc) and squash (squ), which function in germline RNAi processes. Zuc and Squ contain domains with homologies to nucleases. Mutant females are sterile and show dorsoventral patterning defects during oogenesis. In addition, Oskar protein is ectopically expressed in early oocytes, where it is normally silenced by RNAi mechanisms. Zuc and Squ localize to the perinuclear nuage and interact with Aubergine, a PIWI class protein. Mutations in zuc and squ induce the upregulation of Het-A and Tart, two telomere-specific transposable elements, and the expression of Stellate protein in the Drosophila germline. We show that these defects are due to the inability of zuc and squ mutants to produce repeat-associated small interfering RNAs.

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          Author and article information

          Journal
          Developmental Cell
          Developmental Cell
          Elsevier BV
          15345807
          June 2007
          June 2007
          : 12
          : 6
          : 851-862
          Article
          10.1016/j.devcel.2007.03.022
          1945814
          17543859
          01bc32df-7b8b-4abb-8a61-8910f063ce30
          © 2007

          https://www.elsevier.com/tdm/userlicense/1.0/

          https://www.elsevier.com/open-access/userlicense/1.0/

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