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      A long noncoding (lnc)RNA governs expression of the phosphate transporter Pho84 in fission yeast and has cascading effects on the flanking prt lncRNA and pho1 genes

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          Abstract

          The expression of the phosphate transporter Pho84 in fission yeast Schizosaccharomyces pombe is repressed in phosphate-rich medium and induced during phosphate starvation. Two other phosphate-responsive genes in S. pombe ( pho1 and tgp1) had been shown to be repressed in cis by transcription of a long noncoding (lnc) RNA from the upstream flanking gene, but whether pho84 expression is regulated in this manner is unclear. Here, we show that repression of pho84 is enforced by transcription of the SPBC8E4.02c locus upstream of pho84 to produce a lncRNA that we name prt2 ( pho - repressive transcript 2). We identify two essential elements of the prt2 promoter, a HomolD box and a TATA box, mutations of which inactivate the prt2 promoter and de-repress the downstream pho84 promoter under phosphate-replete conditions. We find that prt2 promoter inactivation also elicits a cascade effect on the adjacent downstream prt (lncRNA) and pho1 (acid phosphatase) genes, whereby increased pho84 transcription down-regulates prt lncRNA transcription and thereby de-represses pho1. Our results establish a unified model for the repressive arm of fission yeast phosphate homeostasis, in which transcription of prt2, prt, and nc-tgp1 lncRNAs interferes with the promoters of the flanking pho84, pho1, and tgp1 genes, respectively.

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          Author and article information

          Journal
          J Biol Chem
          J. Biol. Chem
          jbc
          jbc
          JBC
          The Journal of Biological Chemistry
          American Society for Biochemistry and Molecular Biology (11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A. )
          0021-9258
          1083-351X
          23 March 2018
          2 February 2018
          : 293
          : 12
          : 4456-4467
          Affiliations
          From the []Molecular Biology Program, Sloan-Kettering Institute, New York and
          the [§ ]Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065
          Author notes
          [1 ] To whom correspondence may be addressed. Tel.: 212-639-7145; E-mail: s-shuman@ 123456ski.mskcc.org .
          [2 ] To whom correspondence may be addressed. Tel.: 212-746-6518; E-mail: bschwer@ 123456med.cornell.edu .

          Edited by Charles E. Samuel

          Article
          PMC5868275 PMC5868275 5868275 RA117.001352
          10.1074/jbc.RA117.001352
          5868275
          29414789
          0256db7c-a808-487c-b421-83fb5d29da20
          © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
          Funding
          Funded by: HHS | NIH | National Institute of General Medical Sciences (NIGMS) , open-funder-registry 10.13039/100000057;
          Award ID: GM52470
          Categories
          Gene Regulation

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