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      Biophysical characterization of MDR breast cancer cell lines reveals the cytoplasm is critical in determining drug sensitivity.

      Biochimica et Biophysica Acta

      metabolism, Anthracyclines, Antineoplastic Agents, pharmacology, Breast Neoplasms, pathology, Carbocyanines, Cell Line, Tumor, Cell Survival, drug effects, Cisplatin, Colchicine, Cytoplasm, Doxorubicin, Drug Resistance, Multiple, Phenotype, Drug Resistance, Neoplasm, Electrophoresis, methods, Etoposide, Female, Fluorescent Dyes, Humans, Inhibitory Concentration 50, Membrane Potentials, Membrane Transport Proteins, Multidrug Resistance-Associated Proteins, Neoplasm Proteins, P-Glycoprotein, Paclitaxel, ATP-Binding Cassette Transporters

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          Abstract

          Dielectrophoresis (DEP) was used to examine a panel of MCF-7 cell lines comprising parental MCF-7 cells and MDR derivatives: MCF-7TaxR (paclitaxel-resistant, P-glycoprotein (P-gp) positive), MCF-7DoxR (doxorubicin-resistant MRP2 positive) plus MCF-7MDR1 (MDR1 transfected, P-gp positive). MCF-7DoxR and MCF-7MDR1 were broadly cross-resistant to natural product anticancer agents, whereas MCF-7TaxR cells were not, contrary to P-gp expression. Whilst DEP revealed modest membrane changes in MDR sub-lines, we saw significant changes in their cytoplasmic conductivity: MCF-7TaxR

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          Journal
          17270349
          10.1016/j.bbagen.2006.12.002

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