2
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Development of a plasmid display system using GAL4 DNA binding domain for the in vitro screening of functional proteins.

      1 , ,
      Biotechnology letters
      Springer Nature America, Inc

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A plasmid display system using GAL4 DNA binding domain (GAL4 DBD) was constructed to enrich the molecular diversity and in vitro selection of functional proteins. Model proteins used were enhanced green fluorescent protein (EGFP) and glutathione S-transferase (GST). The feasibility of this display system was examined using enrichment experiments of target protein from a model protein mixture and identifying the encoding genes by PCR, in which the model protein mixture includes GAL4 DBD/GST fusion protein, GAL4 DBD/EGFP fusion protein, and xylanase. Target proteins of GAL4 DBD/GST and GAL4 DBD/EGFP from the model protein mixture were efficiently isolated by the plasmid display, respectively. The results show that the display system is sufficiently sensitive to select a target protein from a protein mixture, and that it is possible to discover the functional proteins from large libraries using relatively simple approaches.

          Related collections

          Author and article information

          Journal
          Biotechnol. Lett.
          Biotechnology letters
          Springer Nature America, Inc
          0141-5492
          0141-5492
          Nov 2005
          : 27
          : 21
          Affiliations
          [1 ] School of Chemical and Biological Engineering, Seoul National University, 151-742, Seoul, Korea.
          Article
          10.1007/s10529-005-2735-4
          16247679
          02d3b92f-4855-482b-8190-15389d657d23
          History

          Comments

          Comment on this article