0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Pituitary Nuclear Retention of Dexamethasone and ACTH Biosynthesis

      , ,

      Neuroendocrinology

      S. Karger AG

      CRF, Dexamethasone binding, ACTH biosynthesis, ACTH release

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The possible binding of <sup>3</sup>H-dexamethasone (Dx)to the anterior pituitary and the effect of the steroid on neo-synthesis and release of ACTH of glands incubated with crude CRF extracts were examined. Neosynthesis of ACTH was determined by immunoprecipitation after incorporation of <sup>14</sup>C-phenylalanine into the peptide. Using a cell-free system, <sup>3</sup>H-Dx was shown to combine with the nuclear fraction of the pituitary. Short-term in vitro pre-treatment with Dx suppressed the CRF-induced hormonal release, but did not alter ACTH biosynthesis. The releasing mechanism, on the other hand, did not appear to be related to de novo synthesis. Prolonged in vivo administration of high concentrations of Dx initially caused a decrease of ACTH synthesis and, later, also impaired the synthesis of total proteins. Synthesis and release of ACTH may be dissociated, but there is a possibility that a correlation exists between these two processes. On the other hand and as far as the direct effect of Dx on the pituitary is concerned, the nuclear uptake of the steroid probably reflects an early step in the modulation of corticotropic activity.

          Related collections

          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1974
          1974
          25 March 2008
          : 15
          : 6
          : 365-375
          Affiliations
          Institut de Physiologie, Université Louis Pasteur, Strasbourg, and ERA No. 178 of the CNRS
          Article
          122327 Neuroendocrinology 1974;15:365–375
          10.1159/000122327
          4373679
          © 1974 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 11
          Categories
          Paper

          Comments

          Comment on this article