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      Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template.

      Nucleic Acids Research
      Oxford University Press (OUP)

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          Abstract

          Avian myeloblastosis virus and Maloney murine leukemia virus RNase H-reverse transcriptases pause when they encounter a 2'-5' linkage or a 2'-thiophosphate in their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2'-5' linkage but before the 2'-thiophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.

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          Journal
          7544885
          307115
          10.1093/nar/23.15.2811

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