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      Cell type-specific effects of Rb deletion in the murine retina.

      Genes & development

      Animals, Apoptosis, genetics, Blotting, Western, Crosses, Genetic, Cyclin D1, metabolism, Disease Models, Animal, Genes, Retinoblastoma, Immunoblotting, Immunohistochemistry, In Situ Nick-End Labeling, Mice, Mutation, Nuclear Proteins, Proteins, Retina, embryology, growth & development, pathology, Retinal Ganglion Cells, Retinoblastoma, Retinoblastoma-Like Protein p107, Retinoblastoma-Like Protein p130

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          Abstract

          Certain cells of the human retina are extremely sensitive to loss of function of the retinoblastoma tumor suppressor gene RB. Retinoblastomas develop early in life and at high frequency in individuals heterozygous for a germ-line RB mutation, and sporadic retinoblastomas invariably have somatic mutation in the RB gene. In contrast, retinoblastomas do not develop in Rb+/- mice. Although retinoblastoma is thought to have developmental origins, the function of Rb in retinal development has not been fully characterized. Here we studied the role of Rb in normal retinal development and in retinoblastoma using conditional Rb mutations in the mouse. In late embryogenesis, Rb-deficient retinas exhibited ectopic S-phase and high levels of p53-independent apoptosis, particularly in the differentiating retinal ganglion cell layer. During postnatal retinal development, loss of Rb led to more widespread retinal apoptosis, and adults showed loss of photoreceptors and bipolar cells. Conditional Rb mutation in the retina did not result in retinoblastoma formation even in a p53-mutant background. However, on a p107- or p130-deficient background, Rb mutation in the retina caused retinal dysplasia or retinoblastoma. Copyright 2004 Cold Spring Harbor Laboratory Press ISSN

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          Author and article information

          Journal
          15231717
          478190
          10.1101/gad.1203304

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